{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSE1776GSM30759": {"growth condition": "control"}}, {"GSE1776GSM30759": {"growth condition": "control"}}, {"GSE1776GSM30759": {"growth condition": "control"}}, {"GSE1776GSM30759": {"growth condition": "control"}}, {"GSE1776GSM30774": {"growth condition": "starved"}}, {"GSE1776GSM30774": {"growth condition": "starved"}}, {"GSE1776GSM30774": {"growth condition": "starved"}}], "id": 8435, "ownerprofile_id": "arrayexpress_sid", "platform": 8, "summary_wrapped": "C2C12 mouse myoblasts (American Type Culture Collection, Manassas, VA) were maintained in growth medium (DMEM supplemented with 10% Fetal...", "pubmed_id": 15608089, "geo_gse_id": "E-GEOD-1776", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 7, "tags": ["serum"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-by-array-of-mouse-myotubes", "geo_id_plat": "E-GEOD-1776_A-AFFY-36", "name": "Transcription profiling by array of mouse myotubes grown under control and starvation conditions", "created": "Nov.24, 2014", "summary": "C2C12 mouse myoblasts (American Type Culture Collection, Manassas, VA) were maintained in growth medium (DMEM supplemented with 10% Fetal Bovine serum, Invitrogen Corporation, Carlsbad, CA) in 5% CO2. Cells were plated at a density of 40-50% in 100mm dishes. When cells reached ~90% confluency 24 hours later, they were switched to differentiation media (DMEM containing 2% horse serum; GIBCO, Invitrogen Corporation, Carlsbad, CA) which was subsequently changed every 48 hours. All cells were allowed to differentiate for 4 days with feeding every two days (day 0, 2, and 4). Beginning at day 4, control cells were re-fed every 48 hours whereas experimental cells were not re-fed. After an additional 4 days (8 days post differentiation), cells were harvested and total RNA was isolated from control and starved (non re-fed cells)", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-1776", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-1776/samples/"}