ArrayExpress Uploaderarrayexpress_sidmousewild type, PTIP+1 month oldwild type, PTIP+2 month oldwild type, PTIP+3 month oldwild type, PTIP+4 month oldwild type, PTIP+5 month oldwild type, PTIP+6 month oldwild type, PTIP+7 month oldwild type, PTIP+8 month oldPod-Cre, PTIP-9 month oldPod-Cre, PTIP-10 month oldPod-Cre, PTIP-11 month oldPod-Cre, PTIP-12 month oldPod-Cre, PTIP-13 month oldPod-Cre, PTIP-14 month oldPod-Cre, PTIP-15 month oldPod-Cre, PTIP-16 month oldPod-Cre, PTIP-17 month oldPod-Cre, PTIP-18 month old579006Glomerular RNA comparison between wild-type and podocyte specific deletion of the PTIP gene in 1 month old kidneys. The PTIP gene was...E-GEOD-17709/profile/8773/arrayexpressuploader218proteinDec.12, 2014Falsegene-expression-analysis-of-a-podocyte-specific-ptE-GEOD-17709_A-AFFY-45Gene expression analysis of a podocyte specific PTIP deletion in mouse glomerular preparations at 1 month of ageNov.11, 2014Glomerular RNA comparison between wild-type and podocyte specific deletion of the PTIP gene in 1 month old kidneys. The PTIP gene was deleted using a floxed allele and a Podocin-Cre driver strain. These mice develop protein urea by 3 months of age. This study was designed to find gene expression differences prior to the onset of the phenotype. A ptip floxed allele was crossed into the Podocin-Cre expressing strain to generate ptip fl/fl homozygous mice or ptip fl/- transheterozygous mice with or without the Cre expressing transgene. Animals were genotyped and assigend into two groups: Mutants with PTIP deletion (PTIP-) with the genotype ptip fl/fl:Pod-Cre or ptip fl/-:Pod-Cre or control mice (PTIP+) with either ptip fl/fl or ptip fl/-. Note that the floxed allele produced normal levels of wild-type PTIP protein. Kidneys were excised at 1 month of age. Glomeruli enriched fractions were generated by sieving of the tissue homogenates. RNA was prepared from glomerular enriched fractions.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-17709http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-17709/samples/