{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM437517": {"PIR KNOCK-DOWN": "yes"}}, {"GSM437517": {"PIR KNOCK-DOWN": "yes"}}, {"GSM437519": {"PIR KNOCK-DOWN": "no"}}, {"GSM437519": {"PIR KNOCK-DOWN": "no"}}, {"GSM437519": {"PIR KNOCK-DOWN": "no"}}, {"GSM437519": {"PIR KNOCK-DOWN": "no"}}], "id": 3383, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Pirin (PIR) is a putative transcriptional regulator abundantly expressed in melanocytes and in a subset of primary and metastatic...", "geo_gse_id": "E-GEOD-17551", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 6, "tags": ["cell", "melanoma", "metastatic melanoma"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "genes-regulated-after-transient-knock-down-of-piri", "geo_id_plat": "E-GEOD-17551_A-AFFY-44", "name": "Genes regulated after transient knock-down of Pirin in WM-266 cells", "created": "Sep.12, 2014", "summary": "Pirin (PIR) is a putative transcriptional regulator abundantly expressed in melanocytes and in a subset of primary and metastatic melanomas. Ablation of PIR in the melanoma cell lines results in induction of a senescence-like phenotype.  Keywords: Transcriptional regulation, knock-down using siRNA We analyzed gene expression profiles of WM-266.4 metastatic melanoma cells after knock-down of PIR, which was achieved using a pool of four PIR-siRNA duplexes (siPIR). A pool of four non-targeting siRNA duplexes was used as control of off-target transcriptional effects (siCTR). Cells that underwent transfection with no oligonucleotides (oligofectamine only) were also included to measure all non-specific effects (NO cells). For each sample, an RNA pool was obtained by mixing equal quantities of total RNA from each of three independent RNA extractions. Each biotin-labeled target was hybridized to two GeneChip HG-U133 Plus v.2 arrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-17551", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-17551/samples/"}