{"platform": 6, "owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSE17513GSM436530": {}}, {"GSE17513GSM436534": {}}, {"GSE17513GSM436538": {}}, {"GSE17513GSM436531": {}}, {"GSE17513GSM436535": {}}, {"GSE17513GSM436539": {}}, {"GSE17513GSM436532": {}}, {"GSE17513GSM436536": {}}, {"GSE17513GSM436540": {}}, {"GSE17513GSM436533": {}}, {"GSE17513GSM436537": {}}, {"GSE17513GSM436541": {}}], "id": 5781, "ownerprofile_id": "arrayexpress_sid", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-17513", "summary_wrapped": "Mamamlian cardiogenesis occurs through the development of discreate populations of first and second heart field progenitors.  We have...", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 12, "tags": ["cell", "embryonic stem cell", "heart", "line", "stem cell"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-embryonic-stem-7", "geo_id_plat": "E-GEOD-17513_A-AFFY-45", "name": "Transcription profiling of mouse embryonic stem cell derived cardiac progenitors", "created": "Nov.11, 2014", "summary": "Mamamlian cardiogenesis occurs through the development of discreate populations of first and second heart field progenitors.  We have used a dual transgenic color reproter system to isolate purified populations of these progenitors. We used microarrays to detail the global programme of gene expression underlying cardiac development in mouse; All four populations of cells are derived from embryonic stem cells differentiating in vitro (day 6 of in vitro differentaition).  The stem cell   line has two transgenic reporters as follows:; 1.  The second heart field (SHF) specific reporter of the Mef2C gene (E. Dodou, S. M. Xu, B. L. Black, Mech Dev 120, 1021 (Sep, 2003)) driving the   expression of dsRed; 2.  The cardiac specific enhancer ( C. L. Lien et al., Development 126, 75 (Jan, 1999)) driving the expression of eGFP.   Thus, the red cells are SHF specific, the green cells are cardiac specific, and the red/green are SHF and cardiac  specific.  These cells are compared   to the double negative cells which serve as a control. Experiment Overall Design: Embryonic stem cell derived progenitors were isolated into four distinct populations by FACS purifying these progenitors based on a two color reporter system.  Four populations were then compared to each other by transcriptional profiling.", "geo_gse_id": "E-GEOD-17513", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-17513/samples/"}