{"platform": 6, "owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM434350": {"TREATMENT": "HIV-1"}}, {"GSM434350": {"TREATMENT": "HIV-1"}}, {"GSM434350": {"TREATMENT": "HIV-1"}}, {"GSM434380": {"TREATMENT": "mock virus"}}, {"GSM434380": {"TREATMENT": "mock virus"}}, {"GSM434380": {"TREATMENT": "mock virus"}}], "id": 5773, "ownerprofile_id": "arrayexpress_sid", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-17383", "summary_wrapped": "We present a microarray analysis of primary mouse astrocytes exposed to HIV-1 in culture. Results are compared with previous genomic...", "pubmed_id": 19697136, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 6, "tags": ["cell", "genome"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "toward-a-better-understanding-of-potential-roles-o", "geo_id_plat": "E-GEOD-17383_A-AFFY-45", "name": "Toward a Better Understanding of Potential Roles of Astrocytes in HIV-1-associated Neurocognitive Disorders", "created": "Nov.11, 2014", "summary": "We present a microarray analysis of primary mouse astrocytes exposed to HIV-1 in culture. Results are compared with previous genomic studies of HIV-1 effect in human astrocytes and human and macaque brains. Day-two neonatal mouse astrocytes were exposed in triplicates to cell-free HIV-1/NL4-3 at m.o.i. of 1 and cultured for 24 hours.  As control, cells were treated in triplicates with a mock virus concentrate at the same dilution (vol/vol) as the wild-type virus.  As an example of large scale gene expression analysis we shall provide some details of the method; full description is in Kim et al. (S.-Y. Kim et al., 2004).  Total RNA was prepared from cell cultures using the RNAeasy total RNA extraction kit (Qiagen, CA).  RNA quality was assessed by electrophoresis and spectrophotometric analysis; between 1 and 10 \u00b5g of total RNA was used to generate a cDNA, and then 1 \u00b5g of cDNA product was used in an in vitro transcription reaction that contained biotinylated UTP and CTP.  Twenty \u00b5g of full-length cRNA was fragmented and was subjected to gene expression analysis on the Affymetrix Mouse Genome 430 2.0 Array chip.  Affymetrix software was used to generate CHP files. Significance analysis was performed using ArrayAssist software (Stratagene).", "geo_gse_id": "E-GEOD-17383", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-17383/samples/"}