ArrayExpress Uploader03355memory B cellnaive B cellCD21lo transitional B cellCD21hi transitional B cellnaive B cellCD21lo transitional B cellCD21hi transitional B cellnaive B cellCD21lo transitional B cellCD21hi transitional B cellmemory B cellnaive B cellCD21lo transitional B cellCD21hi transitional B cellarrayexpress_sid4Goals/objectives: to identify various gene expression in B cell subsets derived from human PBMC and cord blood Sample: human PBMC and...19965666E-GEOD-17186/profile/8773/arrayexpressuploader114celltransitionalDec.12, 2014Falsetranscription-profiling-of-human-b-cell-subtypes-dE-GEOD-17186_A-AFFY-44Transcription profiling of human B cell subtypes derived from peripheral blood or umbilical cord blood.Sep.12, 2014Goals/objectives: to identify various gene expression in B cell subsets derived from human PBMC and cord blood Sample: human PBMC and human cord blood Cell subsets: memory B cell (CD20+CD10-CD27+), naive B cell (CD20+CD10-CD27-), CD21lo transitional B cell (CD20+CD10+CD27-CD21lo), CD21hi transitional B cell (CD20+CD10+CD27-CD21hi) Number of sample: PBMC (pooled from 2 donors), cord blood (pooled from 2-3 donors) Number of repeats: 2 Microarray: Affymetrix HU133Plus2 RNA extraction: Trizol (Invitrogen) RNA preparation/hybridization: All according to affymetrix protocolhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-17186humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-17186/samples/