Dataset: Regulatory mechanisms of TSG-6 (TNF-alfa-induced protein-6: Tnfip6)-deficient and wild-type synovial fibroblasts
BALB/c mice are susceptible to proteoglycan (PG) aggrecan-induced arthritis (PGIA), and the absence of TSG-6 further increases...
BALB/c mice are susceptible to proteoglycan (PG) aggrecan-induced arthritis (PGIA), and the absence of TSG-6 further increases susceptibility and local inflammatory reactions, including neutrophil invasion into the joints. To gain insight into the mechanisms of TSG-6 action, synovial fibroblasts were isolated from wild-type and TSG-6-KO mice, cultured and exposed to various agents affecting either the TSG-6 expression and/or modify the intracellular function of TSG-6. In the present microarray studies, we have identified differences in gene expression by fibroblasts isolated from wild-type or TSG-6-KO mice Keywords: Genetic modification In this study we compared the gene expression profile of 42 fibroblast cultures from mouse synovial samples. Fifteen samples were wild-type and 27 samples were TSG-6 knockout. In these two genotype groups fibroblasts were treated with TNFa, LPS, TSG-6, HA, or HA+TSG-6, and were compared to untreated samples in the same genotype group. We also compared the same treatments between KO and WT.
- Species:
- mouse
- Samples:
- 42
- Source:
- E-GEOD-17160
- PubMed:
- 21566135
- Updated:
- Dec.12, 2014
- Registered:
- Nov.11, 2014
Sample | TYPE | TREATMENT |
---|---|---|
GSM429336 | Wild-type | none |
GSM429336 | Wild-type | none |
GSM429336 | Wild-type | none |
GSM429336 | Wild-type | none |
GSM429336 | Wild-type | none |
GSM42934 | Wild-type | TNFa-treated |
GSM42934 | Wild-type | TNFa-treated |
GSM429343 | Wild-type | LPS-treated |
GSM429343 | Wild-type | LPS-treated |
GSM429345 | Wild-type | TSG6-treated |
GSM429345 | Wild-type | TSG6-treated |
GSM429347 | Wild-type | HA-treated |
GSM429347 | Wild-type | HA-treated |
GSM429349 | Wild-type | TSG6+HA-treated |
GSM429349 | Wild-type | TSG6+HA-treated |
GSM42935 | TSG-6 knockout | none |
GSM42935 | TSG-6 knockout | none |
GSM42935 | TSG-6 knockout | none |
GSM42935 | TSG-6 knockout | none |
GSM42935 | TSG-6 knockout | none |
GSM42935 | TSG-6 knockout | none |
GSM42935 | TSG-6 knockout | none |
GSM42935 | TSG-6 knockout | none |
GSM429359 | TSG-6 knockout | TNFa-treated |
GSM429359 | TSG-6 knockout | TNFa-treated |
GSM429359 | TSG-6 knockout | TNFa-treated |
GSM429359 | TSG-6 knockout | TNFa-treated |
GSM429363 | TSG-6 knockout | LPS-treated |
GSM429363 | TSG-6 knockout | LPS-treated |
GSM429363 | TSG-6 knockout | LPS-treated |
GSM429363 | TSG-6 knockout | LPS-treated |
GSM429367 | TSG-6 knockout | TSG6-treated |
GSM429367 | TSG-6 knockout | TSG6-treated |
GSM429367 | TSG-6 knockout | TSG6-treated |
GSM429370 | TSG-6 knockout | HA-treated |
GSM429370 | TSG-6 knockout | HA-treated |
GSM429370 | TSG-6 knockout | HA-treated |
GSM429370 | TSG-6 knockout | HA-treated |
GSM429374 | TSG-6 knockout | TSG6+HA-treated |
GSM429374 | TSG-6 knockout | TSG6+HA-treated |
GSM429374 | TSG-6 knockout | TSG6+HA-treated |
GSM429374 | TSG-6 knockout | TSG6+HA-treated |