{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM428146 1": {}}, {"GSM428147 1": {}}, {"GSM428148 1": {}}, {"GSM428149 1": {}}, {"GSM428150 1": {}}, {"GSM428151 1": {}}, {"GSM428152 1": {}}, {"GSM428153 1": {}}, {"GSM428154 1": {}}, {"GSM428155 1": {}}, {"GSM428156 1": {}}, {"GSM428157 1": {}}, {"GSM428158 1": {}}, {"GSM428159 1": {}}], "id": 3352, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "We generated the transcriptional regulatory footprint of phthalimide neovascular factor 1 (PNF1)\u2014a novel synthetic small molecule that...", "geo_gse_id": "E-GEOD-17119", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 14, "tags": ["serum"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcriptional-profiling-of-a-novel-pro-angiogeni", "geo_id_plat": "E-GEOD-17119_A-AFFY-44", "name": "Transcriptional profiling of a novel pro-angiogenic small molecule phthalimide neovascular factor 1 (PNF1)", "created": "Sep.12, 2014", "summary": "We generated the transcriptional regulatory footprint of phthalimide neovascular factor 1 (PNF1)\u2014a novel synthetic small molecule that exhibits significant in vitro endothelial potency and significant in vivo microvascular network expansion\u2014by performing comparative microarray analysis on PNF1-stimulated (versus control) human microvascular endothelial cells (HMVEC) spanning 1-48 h post-supplementation.  We subsequently applied network analysis tools (including substantial libraries of information regarding known associations among network components) to elucidate key signaling components and pathways involved in the PNF1 mechanism-of-action.  We identified that PNF1 first induces function of the tumor necrosis factor-alpha (TNF-\u03b1) signaling pathway, which in turn affects transforming growth factor-beta (TGF-\u03b2) signaling. HMVEC (Cambrex, Walkersville, MD, USA) were cultured in endothelial growth medium 2-microvascular (bulletkit, BioWhittaker, Walkersville, MD, USA) supplemented as directed with 5% fetal bovine serum. The cells (passage 9) were plated at 2.5 x 104 cells/cm2 at 37 degrees Celsius in a humidified chamber with 5% carbon dioxide. They were grown to confluence. After confluence, medium was refreshed, and 30 \u00b5M PNF1 or 0.6% dimethyl sulfoxide (DMSO) vehicle control was added to the sample. Total RNA from the PNF1 (n=1 at each timepoint) and control (n=1 at each timepoint) samples was isolated 1, 2, 4, 8, 16, 24 and 48 h post-supplementation using an RNeasy kit (Qiagen, Inc., Valencia, CA, USA) according to the manufacturer's protocol.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-17119", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-17119/samples/"}