ArrayExpress Uploaderarrayexpress_sidhumanday 14 in culture PRAMEday 14 in culture PRAMEday 14 in culture controlday 14 in culture controlday 7 in culture PRAMEday 7 in culture PRAMEday 7 in culture controlday 7 in culture controlday 4 in culture PRAMEday 4 in culture PRAMEday 4 in culture controlday 4 in culture control139403To identify gene expression that distinguishes hematopoietic cells that express PRAME from those that do not, normal CD34+ cells with...19625708E-GEOD-17100/profile/8773/arrayexpressuploader312Dec.12, 2014Falsegene-expression-associated-with-lentiviral-expressE-GEOD-17100_A-AFFY-33Gene expression associated with lentiviral expression of PRAME in normal hematopoietic progenitors.Jun.19, 2014To identify gene expression that distinguishes hematopoietic cells that express PRAME from those that do not, normal CD34+ cells with forced PRAME expression were compared to cells without PRAME expression in culture over time (days 4, 7, 14) using Affymetrix HU-133A microarrays Normal CD34+ progenitor cells not expressing PRAME were transduced with either PRAME cDNA (no 3' utr) or a corresponding empty vector control by lentiviral vector infection. Two replicates were performed for each condition (PRAME and control) at three experimental time points (days 4, 7, and 14 in culture). A single-stranded linear amplification protocol was used to process total RNA (one microgram) for the microarrays. This protocols is described in detail in PMID 14706461.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-17100http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-17100/samples/