ArrayExpress Uploader05745non stimulatedWild Typenon stimulatedWild Typenon stimulatedWild Typenon stimulatedEFLmDREAMnon stimulatedEFLmDREAMnon stimulatedEFLmDREAMstimulated with LPS and mouse recombinant IL-4 (day 2)Wild Typestimulated with LPS and mouse recombinant IL-4 (day 2)Wild Typestimulated with LPS and mouse recombinant IL-4 (day 2)Wild Typestimulated with LPS and mouse recombinant IL-4 (day 2)EFLmDREAMstimulated with LPS and mouse recombinant IL-4 (day 2)EFLmDREAMstimulated with LPS and mouse recombinant IL-4 (day 2)EFLmDREAMarrayexpress_sid6DREAM/KChIP-3 is a calcium-dependent transcriptional repressor highly expressed in immune cells. Transgenic mice expressing a dominant...21059893E-GEOD-16874/profile/8773/arrayexpressuploader212cellclassgenomeimmunoglobulinplasma cellserumDec.12, 2014Falseexpression-in-wild-type-and-tgdream-mouse-b-cellsE-GEOD-16874_A-AFFY-45Expression in wild type and TgDREAM mouse B cells unstimulated or 2 days after LPS+IL4 stimulationNov.11, 2014DREAM/KChIP-3 is a calcium-dependent transcriptional repressor highly expressed in immune cells. Transgenic mice expressing a dominant active DREAM mutant show reduced serum immunoglobulin levels. In vitro assays show that reduced immunoglobulin secretion is an intrinsic defect of transgenic B cells that occurs without impairment in plasma cell differentiation but with an accelerated entry in cell division and an increase in class switch recombination. B cells from DREAM knockout mice did not show any phenotype, due to compensation by endogenous KChIP-2. Expression arrays revealed modified expression of Edem1 and Derlin3, two proteins related to the ER-associated degradation pathway and of Klf9, a cell-cycle regulator. Our results disclose a function of DREAM and KChIP-2 in Ig subclass production in B lymphocytes. We used Affymetrix microarrays (GeneChip Mouse Genome 430 2.0) to compare global gene expression in wild type (WT) versus transgenic B cells (Tg), unstimulated and 2 days after LPS + IL4 stimulation. For ech type of sample three hybridizations were carried-out (independent biological replicates).http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16874mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16874/samples/