{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 5745, "factors": [{"GSM422913": {"STIMULATION": "non stimulated", "GENOTYPE": "Wild Type"}}, {"GSM422913": {"STIMULATION": "non stimulated", "GENOTYPE": "Wild Type"}}, {"GSM422913": {"STIMULATION": "non stimulated", "GENOTYPE": "Wild Type"}}, {"GSM422916": {"STIMULATION": "non stimulated", "GENOTYPE": "EFLmDREAM"}}, {"GSM422916": {"STIMULATION": "non stimulated", "GENOTYPE": "EFLmDREAM"}}, {"GSM422916": {"STIMULATION": "non stimulated", "GENOTYPE": "EFLmDREAM"}}, {"GSM422919": {"STIMULATION": "stimulated with LPS and mouse recombinant IL-4 (day 2)", "GENOTYPE": "Wild Type"}}, {"GSM422919": {"STIMULATION": "stimulated with LPS and mouse recombinant IL-4 (day 2)", "GENOTYPE": "Wild Type"}}, {"GSM422919": {"STIMULATION": "stimulated with LPS and mouse recombinant IL-4 (day 2)", "GENOTYPE": "Wild Type"}}, {"GSM422922": {"STIMULATION": "stimulated with LPS and mouse recombinant IL-4 (day 2)", "GENOTYPE": "EFLmDREAM"}}, {"GSM422922": {"STIMULATION": "stimulated with LPS and mouse recombinant IL-4 (day 2)", "GENOTYPE": "EFLmDREAM"}}, {"GSM422922": {"STIMULATION": "stimulated with LPS and mouse recombinant IL-4 (day 2)", "GENOTYPE": "EFLmDREAM"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "DREAM/KChIP-3 is a calcium-dependent transcriptional repressor highly expressed in immune cells. Transgenic mice expressing a dominant...", "pubmed_id": 21059893, "geo_gse_id": "E-GEOD-16874", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 12, "tags": ["cell", "class", "genome", "immunoglobulin", "plasma cell", "serum"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-in-wild-type-and-tgdream-mouse-b-cells", "geo_id_plat": "E-GEOD-16874_A-AFFY-45", "name": "Expression in wild type and TgDREAM mouse B cells unstimulated or 2 days after  LPS+IL4 stimulation", "created": "Nov.11, 2014", "summary": "DREAM/KChIP-3 is a calcium-dependent transcriptional repressor highly expressed in immune cells. Transgenic mice expressing a dominant active DREAM mutant show reduced serum immunoglobulin levels. In vitro assays show that reduced immunoglobulin secretion is an intrinsic defect of transgenic B cells that occurs without impairment in plasma cell differentiation but with an accelerated entry in cell division and an increase in class switch recombination. B cells from DREAM knockout mice did not show any phenotype, due to compensation by endogenous KChIP-2. Expression arrays revealed modified expression of Edem1 and Derlin3, two proteins related to the ER-associated degradation pathway and of Klf9, a cell-cycle regulator. Our results disclose a function of DREAM and KChIP-2 in Ig subclass production in B lymphocytes. We used Affymetrix microarrays (GeneChip Mouse Genome 430 2.0) to compare global gene expression in wild type (WT) versus transgenic B cells (Tg), unstimulated and 2 days after LPS + IL4 stimulation. For ech type of sample three hybridizations were carried-out (independent biological replicates).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16874", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16874/samples/"}