{"platform": 6, "owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSE16705GSM418546": {}}, {"GSE16705GSM418547": {}}, {"GSE16705GSM418548": {}}, {"GSE16705GSM418558": {}}, {"GSE16705GSM418559": {}}, {"GSE16705GSM418560": {}}, {"GSE16705GSM418561": {}}, {"GSE16705GSM418562": {}}, {"GSE16705GSM418563": {}}, {"GSE16705GSM418564": {}}, {"GSE16705GSM418565": {}}, {"GSE16705GSM418566": {}}, {"GSE16705GSM418576": {}}, {"GSE16705GSM418577": {}}, {"GSE16705GSM418578": {}}, {"GSE16705GSM418579": {}}, {"GSE16705GSM418589": {}}, {"GSE16705GSM418590": {}}, {"GSE16705GSM418591": {}}, {"GSE16705GSM418592": {}}, {"GSE16705GSM418602": {}}, {"GSE16705GSM418603": {}}, {"GSE16705GSM418604": {}}, {"GSE16705GSM418605": {}}], "id": 5737, "ownerprofile_id": "arrayexpress_sid", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16705", "summary_wrapped": "Transcriptional profiles of Fz4-/- retinal endothelial cells were compared to that of wild type endothelial cells under various culture...", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 24, "tags": ["cell", "endothelial cell", "kidney", "line"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-frizzled4-signali", "geo_id_plat": "E-GEOD-16705_A-AFFY-45", "name": "Transcription profiling of mouse Frizzled4 signaling in cultured retinal endothelial cells", "created": "Nov.11, 2014", "summary": "Transcriptional profiles of Fz4-/- retinal endothelial cells were compared to that of wild type endothelial cells under various culture conditions. The goal was to identify the transcriptional response to Frizzled 4 signaling in cultured retinal endothelial cells. To analyze the Norrin response of WT and Fz4-/- retinal endothelial cells in culture, we co-cultured these cells either with HEK293 cell line that stably expresses Norrin or with control 293 cells. Experiment Overall Design: To isolate the requisite retinal endothelial cell lines, we crossed Fz4-/- and control animals into the Immorto-mouse line in which a gamma interferon responsive H2Kb promoter directs the expression of a temperature sensitive SV40 large T-antigen (Jat et al., 1991). When cultured at 33\u00b0C in the presence of gamma interferon, this conditional oncogene system extends the proliferative capacity of a variety of cell types, which can be subsequently analyzed under phenotypically reverting conditions (37\u00b0C without gamma interferon). The cell immuno-purification procedure follows that described by Matsubara et al. (2000) and Su et al. (2003). The purified cells were then cultured and passaged at 33\u00b0C on dishes coated with 1% gelatin. For RNA preparation, cells were cultured at 37\u00b0C in the absence of interferon gamma. For the Matrigel experiments, endothelial cells previously starved for 24 hrs in DMEM with 1% FBS were trypsinized and plated on the Matrigel layer. The cells were incubated in DMEM with 1% FBS for 6 hours at 37\u00b0C before harvesting for RNA extraction. For the 293 cell co-culture experiments, endothelial cells were co-cultured with a human embryonic kidney (HEK) 293 cell line that stably expresses Norrin, or with control 293 cells.", "geo_gse_id": "E-GEOD-16705", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16705/samples/"}