ArrayExpress Uploader0mouse5736arrayexpress_sid6To characterize the long-term effect on the transcriptome of a decrement in Norrin/Fz4/Lrp signaling, microarray hybridization was...E-GEOD-16703/profile/8773/arrayexpressuploader015cellDec.12, 2014Falsetranscription-profiling-of-mouse-decrement-in-norrE-GEOD-16703_A-AFFY-45Transcription profiling of mouse decrement in Norrin/Frizzled4/Lrp signaling in retinal endothelial cellsNov.11, 2014To characterize the long-term effect on the transcriptome of a decrement in Norrin/Fz4/Lrp signaling, microarray hybridization was performed with RNA from acutely dissociated and anti-PECAM immunoaffinity-purified adult WT, Fz4-/-, Lrp5-/-, and Norrin- retinal vascular cells. Experiment Overall Design: Retinal endothelial cells from 5-10 adult mice were pooled for each sample, the cell immuno-purification procedure follows that described by Matsubara et al. (2000) and Su et al. (2003). 3 replicates of each mutant and 6 replicates of control wild type animals were analyzed.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16703http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16703/samples/