{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 2328, "factors": [{"GSM414107": {"CLONE": "c8"}}, {"GSM414107": {"CLONE": "c8"}}, {"GSM414107": {"CLONE": "c8"}}, {"GSM414107": {"CLONE": "c8"}}, {"GSM414107": {"CLONE": "c8"}}, {"GSM414107": {"CLONE": "c8"}}, {"GSM414107": {"CLONE": "c8"}}, {"GSM414107": {"CLONE": "c8"}}, {"GSM414099": {"CLONE": "c6"}}, {"GSM414099": {"CLONE": "c6"}}, {"GSM414099": {"CLONE": "c6"}}, {"GSM414099": {"CLONE": "c6"}}, {"GSM414099": {"CLONE": "c6"}}, {"GSM414099": {"CLONE": "c6"}}, {"GSM414099": {"CLONE": "c6"}}, {"GSM414099": {"CLONE": "c6"}}, {"GSM414099": {"CLONE": "c6"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Migratory embryonal neuroblasts give rise to several tissues, including the sympathetic nervous system (SNS). Neuroblastomas are...", "pubmed_id": 23649002, "geo_gse_id": "E-GEOD-16477", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 17, "tags": ["cell", "nervous system", "neuroblastoma", "notch", "peripheral", "sympathetic nervous system"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "notch3-is-a-master-regulator-of-motility-in-neurob", "geo_id_plat": "E-GEOD-16477_A-AFFY-44", "name": "NOTCH3 is a master-regulator of motility in neuroblastoma and is essential for cell survival", "created": "Jul.12, 2014", "summary": "Migratory embryonal neuroblasts give rise to several tissues, including the sympathetic nervous system (SNS). Neuroblastomas are paediatric tumours of the peripheral SNS with a highly variable prognosis. We observed that high NOTCH3 expression in neuroblastomas correlated with a poor prognosis. Expression of a NOTCH3 transgene in neuroblastoma cells induced many motility genes and conferred a highly motile phenotype. Expression of these motility genes strongly correlated with NOTCH3 expression in neuroblastomas and many other tumours, suggesting a general role for NOTCH3 in regulation of these genes. Silencing of NOTCH3 or genes of the Notch-processing \u03b3-secretase complex induced apoptosis in all neuroblastoma cell lines tested. These data suggest that NOTCH3 is a key-regulator of motility, and indispensable for survival of neuroblastoma cells. Time-course experiments of NOTCH3IC induction in two independent clones (c6 and c8), with 9 or 8 time points, respectively.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16477", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16477/samples/"}