{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM413994": {"CELL TYPE": "Lower-SP HSC"}}, {"GSM413994": {"CELL TYPE": "Lower-SP HSC"}}, {"GSM413994": {"CELL TYPE": "Lower-SP HSC"}}, {"GSM413997": {"CELL TYPE": "Upper-SP HSC"}}, {"GSM413997": {"CELL TYPE": "Upper-SP HSC"}}, {"GSM413997": {"CELL TYPE": "Upper-SP HSC"}}], "id": 5714, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "The traditional view of hematopoiesis has been that all the cells of the peripheral blood are the progeny of a unitary homogeneous pool...", "geo_gse_id": "E-GEOD-16475", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 6, "tags": ["hematopoietic system", "peripheral"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-from-side-population-subfraction-h", "geo_id_plat": "E-GEOD-16475_A-AFFY-45", "name": "Expression data from side population subfraction hematopoietic stem cells", "created": "Nov.11, 2014", "summary": "The traditional view of hematopoiesis has been that all the cells of the peripheral blood are the progeny of a unitary homogeneous pool of hematopoietic stem cells (HSCs).  Recent evidence suggests that the hematopoietic system is actually maintained by a consortium of HSC subtypes with distinct functional characteristics.  We show here that myeloid-biased HSCs (My-HSCs) and lymphoid-biased (Ly-HSCs) can be purified according to their capacity for Hoechst dye efflux in combination with canonical HSC markers. We used microarray expression profiling to determine the transcriptional profiles of myeloid-biased lower-SP HSCs and lymphoid-biased upper-SP HSCs Three biological replicates were analyzed for each HSC subpopulation.  Lower-SP and upper-SP HSCs were purified from three pools of mice on separate days.  HSCs were further purified with the addition of canonical HSC makers; Sca-1+ c-Kit+ Lineage-", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16475", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16475/samples/"}