{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 3302, "factors": [{"GSM410843": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD207 positive", "organism part": "Langerhans cell histiocytosis lesion"}}, {"GSM410843": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD207 positive", "organism part": "Langerhans cell histiocytosis lesion"}}, {"GSM410845": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD207 positive", "organism part": "  "}}, {"GSM410843": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD207 positive", "organism part": "Langerhans cell histiocytosis lesion"}}, {"GSM410843": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD207 positive", "organism part": "Langerhans cell histiocytosis lesion"}}, {"GSM410843": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD207 positive", "organism part": "Langerhans cell histiocytosis lesion"}}, {"GSM410843": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD207 positive", "organism part": "Langerhans cell histiocytosis lesion"}}, {"GSM410843": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD207 positive", "organism part": "Langerhans cell histiocytosis lesion"}}, {"GSM410843": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD207 positive", "organism part": "Langerhans cell histiocytosis lesion"}}, {"GSM410843": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD207 positive", "organism part": "Langerhans cell histiocytosis lesion"}}, {"GSM410843": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD207 positive", "organism part": "Langerhans cell histiocytosis lesion"}}, {"GSM410843": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD207 positive", "organism part": "Langerhans cell histiocytosis lesion"}}, {"GSM410843": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD207 positive", "organism part": "Langerhans cell histiocytosis lesion"}}, {"GSM410938": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD3 positive", "organism part": "Langerhans cell histiocytosis lesion"}}, {"GSM410938": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD3 positive", "organism part": "Langerhans cell histiocytosis lesion"}}, {"GSM410938": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD3 positive", "organism part": "Langerhans cell histiocytosis lesion"}}, {"GSM410938": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD3 positive", "organism part": "Langerhans cell histiocytosis lesion"}}, {"GSM410938": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD3 positive", "organism part": "Langerhans cell histiocytosis lesion"}}, {"GSM410938": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD3 positive", "organism part": "Langerhans cell histiocytosis lesion"}}, {"GSM410938": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD3 positive", "organism part": "Langerhans cell histiocytosis lesion"}}, {"GSM410938": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD3 positive", "organism part": "Langerhans cell histiocytosis lesion"}}, {"GSM410938": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD3 positive", "organism part": "Langerhans cell histiocytosis lesion"}}, {"GSM410938": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD3 positive", "organism part": "Langerhans cell histiocytosis lesion"}}, {"GSM410938": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD3 positive", "organism part": "Langerhans cell histiocytosis lesion"}}, {"GSM410938": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD3 positive", "organism part": "Langerhans cell histiocytosis lesion"}}, {"GSM41114": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD3 positive", "organism part": "peripheral blood"}}, {"GSM41114": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD3 positive", "organism part": "peripheral blood"}}, {"GSM41114": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD3 positive", "organism part": "peripheral blood"}}, {"GSM41114": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD3 positive", "organism part": "peripheral blood"}}, {"GSM41114": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD3 positive", "organism part": "peripheral blood"}}, {"GSM41114": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD3 positive", "organism part": "peripheral blood"}}, {"GSM41114": {"disease state": "Langerhans cell histiocytosis", "cell type": "CD3 positive", "organism part": "peripheral blood"}}, {"GSM411150": {"disease state": "normal", "cell type": "CD207 positive", "organism part": "skin"}}, {"GSM411150": {"disease state": "normal", "cell type": "CD207 positive", "organism part": "skin"}}, {"GSM411150": {"disease state": "normal", "cell type": "CD207 positive", "organism part": "skin"}}, {"GSM411150": {"disease state": "normal", "cell type": "CD207 positive", "organism part": "skin"}}, {"GSM411150": {"disease state": "normal", "cell type": "CD207 positive", "organism part": "skin"}}, {"GSM411150": {"disease state": "normal", "cell type": "CD207 positive", "organism part": "skin"}}, {"GSM411150": {"disease state": "normal", "cell type": "CD207 positive", "organism part": "skin"}}, {"GSM411150": {"disease state": "normal", "cell type": "CD207 positive", "organism part": "skin"}}, {"GSM411150": {"disease state": "normal", "cell type": "CD207 positive", "organism part": "skin"}}, {"GSM411150": {"disease state": "normal", "cell type": "CD207 positive", "organism part": "skin"}}, {"GSM411150": {"disease state": "normal", "cell type": "CD207 positive", "organism part": "skin"}}, {"GSM411150": {"disease state": "normal", "cell type": "CD207 positive", "organism part": "skin"}}, {"GSM411663": {"disease state": "normal", "cell type": "CD3 positive", "organism part": "tonsil"}}, {"GSM411663": {"disease state": "normal", "cell type": "CD3 positive", "organism part": "tonsil"}}, {"GSM411663": {"disease state": "normal", "cell type": "CD3 positive", "organism part": "tonsil"}}, {"GSM411663": {"disease state": "normal", "cell type": "CD3 positive", "organism part": "tonsil"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Langerhans-cell histiocytosis (LCH) is characterized by heterogeneous lesions containing CD207+ Langerhans cells (LCs) and lymphocytes....", "pubmed_id": 20220088, "geo_gse_id": "E-GEOD-16395", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 48, "tags": ["bone", "cell", "cell phenotype", "dendritic", "histiocytosis", "langerhans-cell histiocytosis", "peripheral", "skin", "tonsil"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcrition-profiling-of-langerhans-cell-histiocy", "geo_id_plat": "E-GEOD-16395_A-AFFY-44", "name": "Transcrition profiling of  Langerhans Cell Histiocytosis to determine cell specific gene expression", "created": "Sep.12, 2014", "summary": "Langerhans-cell histiocytosis (LCH) is characterized by heterogeneous lesions containing CD207+ Langerhans cells (LCs) and lymphocytes.   In this study, we isolated CD207+ cells and CD3+ T cells from LCH lesions to determine cell-specific gene expression.  Compared to control epidermal CD207+ cells, the LCH CD207+ cells yielded 2113 differentially-expressed genes (FDR<0.01).  Surprisingly, expression of many genes previously associated with LCH, including cell-cycle regulators, pro-inflammatory cytokines and chemokines were not significantly different from control LCs in our study.  However, several novel genes whose products activate and recruit T cells to sites of inflammation, including SPP1 (osteopontin), were highly over-expressed in LCH CD207+ cells.  Furthermore, several genes associated with immature myeloid dendritic cells were over-expressed in LCH CD207+ cells.  Compared to the peripheral CD3+ cells from LCH patients, the LCH lesion CD3+ cells yielded only 162 differentially-regulated genes (FDR<0.01), and the expression profile of the LCH lesion CD3+ cells was consistent with an activated regulatory T cell phenotype with increased expression of FOXP3, CTLA4 as well as SPP1.  Based on these results, we propose a new model of LCH pathogenesis in which lesions do not arise from epidermal Langerhans cells, but from accumulation of bone-marrow derived immature myeloid dendritic cells that recruit activated lymphocytes. Cell-specific gene expression from LCH biopsy specimens was evaluated by comparing hybridization signal from amplified cDNA on Affymetrix gene chips (U133A Plus 2.0) (Table1).  Three sets of comparisons were performed: 1) Thirteen LCH CD207+ samples were compared to 12 control skin LCH CD207+ samples. 2) Seven LCH lesion CD3+ samples were compared to 7 peripheral blood CD3+ samples from the same patients.  3) Twelve LCH lesion CD3+ samples were compared to 4 pooled control tonsil CD3+ samples (5 individual tonsil CD3+ samples/pool).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16395", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16395/samples/"}