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Home › Dataset Library › Expression data from human alternatively activated macrophages

Dataset: Expression data from human alternatively activated macrophages

Human CD14 positive monocytes were purified from healthy volunteers’ blood and cultured in vitro for 6 hours. While culturing,...

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Human CD14 positive monocytes were purified from healthy volunteers’ blood and cultured in vitro for 6 hours. While culturing, macrophages were activated alternatively with interleukin-4 (IL-4 100 ng/ml). Simultaneously, macrophages were also treated with vehicle (DMSO:ethanol) or 1uM synthetic PPARg agonist, Rosiglitazone. We used Affymetrix microarrays (U133Plus 2.0) to analyze activation and PPARg-induced gene expression changes. Monocytes from 2 donors were used and treated as indicated in Summary. Technical replicates were generated from each sample.

Species:
human

Samples:
8

Source:
E-GEOD-16386

PubMed:
21093321

Updated:
Dec.12, 2014

Registered:
Sep.12, 2014


Factors: (via ArrayExpress)
Sample DONOR TREATMENT
GSM411223 1 control
GSM411223 1 control
GSM411225 1 rosiglitazone
GSM411225 1 rosiglitazone
GSM411227 2 control
GSM411227 2 control
GSM411229 2 rosiglitazone
GSM411229 2 rosiglitazone

Tags

  • interleukin
  • interleukin-4

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