ArrayExpress Uploader032991NoneNonemonocyte0h1controlcontrolmacrophage4h1controlrosiglitazonemacrophage4h1IL-4controlmacrophage4h1IL-4rosiglitazonemacrophage4h1IFNg+TNFcontrolmacrophage4h1IFNg+TNFrosiglitazonemacrophage4h1controlcontrolmacrophage12h1controlrosiglitazonemacrophage12h1IL-4controlmacrophage12h1IL-4rosiglitazonemacrophage12h1IFNg+TNFcontrolmacrophage12h1IFNg+TNFrosiglitazonemacrophage12h2NoneNonemonocyte0h2controlcontrolmacrophage12h2controlrosiglitazonemacrophage12h2IL-4controlmacrophage12h2IL-4rosiglitazonemacrophage12h2IFNg+TNFcontrolmacrophage12h2IFNg+TNFrosiglitazonemacrophage12h2controlcontrolmacrophage24h2controlrosiglitazonemacrophage24h2IL-4controlmacrophage24h2IL-4rosiglitazonemacrophage24h2IFNg+TNFcontrolmacrophage24h2IFNg+TNFrosiglitazonemacrophage24h3controlcontrolmacrophage12h3controlrosiglitazonemacrophage12h3IL-4controlmacrophage12h3IL-4rosiglitazonemacrophage12h3IFNg+TNFcontrolmacrophage12h3IFNg+TNFrosiglitazonemacrophage12h3controlcontrolmacrophage72h3controlrosiglitazonemacrophage72h3IL-4controlmacrophage72h3IL-4rosiglitazonemacrophage72h3IFNg+TNFcontrolmacrophage72h3IFNg+TNFrosiglitazonemacrophage72harrayexpress_sid4Human CD14 positive monocytes were purified from healthy volunteers’ blood and cultured in vitro for 4, 12, 24, 72 hours. While...21093321E-GEOD-16385/profile/8773/arrayexpressuploader538interleukininterleukin-4leftDec.12, 2014FalseE-GEOD-16385_A-AFFY-44expression-data-from-human-macrophagesExpression data from human macrophagesSep.12, 2014Human CD14 positive monocytes were purified from healthy volunteers’ blood and cultured in vitro for 4, 12, 24, 72 hours. While culturing, macrophages were activated alternatively with interleukin-4 (IL-4 100 ng/ml) or classically with interferon-gamma (IFNg 100 ng/ml)+tumor necrosis factor (TNF 50 ng/ml) or left without activation. Simultaneously, macrophages were also treated with vehicle (DMSO:ethanol) or 1mM synthetic PPARg agonist, Rosiglitazone. We used Affymetrix microarrays (U133Plus 2.0) to analyze activation and PPARg-induced gene expression changes. Monocytes from 3 donors were used and treated as indicated in Summary.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16385humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16385/samples/