{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 5705, "factors": [{"GSM409423": {"GENOTYPE": "PTIP -/-"}}, {"GSM409423": {"GENOTYPE": "PTIP -/-"}}, {"GSM409425": {"GENOTYPE": "PTIP +/+"}}, {"GSM409425": {"GENOTYPE": "PTIP +/+"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "PPARg and C/EBPa cooperate to control preadipocyte differentiation (adipogenesis). However, the factors that regulate PPARg and C/EBPa...", "pubmed_id": 19583951, "geo_gse_id": "E-GEOD-16263", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 4, "tags": ["adipose tissue", "brown adipose tissue", "histone", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "ptip-regulated-genes-in-mef", "geo_id_plat": "E-GEOD-16263_A-AFFY-45", "name": "PTIP-regulated genes in MEF", "created": "Nov.11, 2014", "summary": "PPARg and C/EBPa cooperate to control preadipocyte differentiation (adipogenesis). However, the factors that regulate PPARg and C/EBPa expression during adipogenesis remain largely unclear. Here we show PTIP, a protein that associates with histone H3K4 methyltransferases, regulates PPARg and C/EBPa expression in mouse embryonic fibroblasts (MEFs) and during preadipocyte differentiation. PTIP deletion in MEFs leads to marked decreases of PPARg expression and PPARg-stimulated C/EBP\u03b1 expression. Further, PTIP is essential for induction of PPARg and C/EBPa expression during preadipocyte differentiation. Deletion of PTIP impairs the enrichment of H3K4 trimethylation and RNA polymerase II on PPARg and C/EBPa promoters. Accordingly, PTIP-/- MEFs and preadipocytes all show striking defects in adipogenesis. Furthermore, rescue of the adipogenesis defect in PTIP-/- MEFs requires co-expression of PPARg and C/EBPa. Finally, deletion of PTIP in brown adipose tissue significantly reduces tissue weight in mice. Thus, by regulating PPARg and C/EBPa expression, PTIP plays a critical role in adipogenesis. To identify PTIP-regulated genes, immortalized PTIP conditional knockout PTIPflox/flox MEFs were infected with retroviruses expressing either Cre recombinase or vector alone. We prepared duplicated RNAs from either vector or Cre infected cells (PTIP+/+ or PTIP-/-) for 4 affymetrix microarrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16263", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16263/samples/"}