ArrayExpress Uploader0humanAirAirAirAirAirCarbon Monoxide Gas (250 ppm)Carbon Monoxide Gas (250 ppm)Carbon Monoxide Gas (250 ppm)Carbon Monoxide Gas (250 ppm)Carbon Monoxide Gas (250 ppm)LPS (1 ug/ml) and Carbon Monoxide (250 ppm)LPS (1 ug/ml) and Carbon Monoxide (250 ppm)LPS (1 ug/ml) and Carbon Monoxide (250 ppm)LPS (1 ug/ml) and Carbon Monoxide (250 ppm)LPS (1 ug/ml) and Carbon Monoxide (250 ppm)LPS (1 ug/ml) and AirLPS (1 ug/ml) and AirLPS (1 ug/ml) and AirLPS (1 ug/ml) and AirLPS (1 ug/ml) and Air3289arrayexpress_sid4This study investigated the global effect of carbon monoxide (CO) gas (250 ppm) on LPS- induced gene expression in THP-1 cells. CO...E-GEOD-16193/profile/8773/arrayexpressuploader120cellgaslineDec.12, 2014Falseinterfering-with-proximal-tlr4-to-nf-kappab-signalE-GEOD-16193_A-AFFY-44Interfering with Proximal TLR4 to NF-kappaB Signal Transduction in Human MonocytesSep.12, 2014This study investigated the global effect of carbon monoxide (CO) gas (250 ppm) on LPS- induced gene expression in THP-1 cells. CO predominantly suppresses LPS-induced gene response. Of the CO-suppressed genes, 18% were transcription factors and most others were cytokines, chemokines and immune response genes. Sequence analysis B binding siteskappafound that 81% of the gene promoters have putative NF- These results suggest that CO inhibits LPS-induced inflammatory B signaling pathwayresponse through regulating NF- The microarrays were performed on THP-1 cells, a human monocytic cell line. Cells were treated with or without LPS (1 µg/ml) in presence or absence of carbon monoxide (250 ppm) for 1 h. Total RNA was isolated, reverse transcribed, labeled and hybridized to oligonucleotide microarrays.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16193http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16193/samples/