{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 3281, "factors": [{"GSM403595 1": {}}, {"GSM403596 1": {}}, {"GSM403597 1": {}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "The origin and function of human double negative (DN) TCR-alpha/beta T cells is unknown.  They are thought to contribute to the...", "pubmed_id": 19734235, "geo_gse_id": "E-GEOD-16130", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 3, "tags": ["cell", "cytokine", "lupus erythematosus", "peripheral", "systemic lupus erythematosus"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "gene-expression-of-tcr-alphabeta-cd4-cd8-human-t-c", "geo_id_plat": "E-GEOD-16130_A-AFFY-44", "name": "Gene expression of TCR-alpha/beta CD4- CD8- human T cells", "created": "Sep.12, 2014", "summary": "The origin and function of human double negative (DN) TCR-alpha/beta T cells is unknown.  They are thought to contribute to the pathogenesis of systemic lupus erythematosus because they expand and accumulate in inflamed organs.  Here we provide evidence that human TCR-alpha/beta CD4- CD8- DN T cells derive exclusively from activated CD8+ T cells.  Freshly isolated TCR-alpha/beta DN T cells display a distinct gene expression and cytokine production profile.  DN cells isolated from peripheral blood as well as DN cells derived in vitro from CD8+ T cells, produce a defined array of pro-inflammatory mediators that includes IL-1, IL-17, IFN-gama, CXCL3, and CXCL2.  These results indicate that, upon activation, CD8+ T cells have the capacity to acquire a distinct phenotype that grants them inflammatory capacity. TCR-alpha-beta+ CD25- T cells from healthy human individuals were sorted into CD4+, CD8+, and CD4-CD8- T cells. Cell lysis and RNA extraction was performed immediately.  RNA from each cell subset was pooled.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16130", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16130/samples/"}