ArrayExpress Uploaderarrayexpress_sidmouse569906The purpose was to determine AcP- and AcPb-dependent gene responses to IL-1 by virally-reconstituting AcP-deficient mouse embryonic...E-GEOD-16104/profile/8773/arrayexpressuploader024Dec.12, 2014Falsetranscription-profiling-of-mouse-il-1b-responses-iE-GEOD-16104_A-AFFY-45Transcription profiling of mouse IL-1b responses in receptor-reconstituted AcP-deficient neuronsNov.11, 2014The purpose was to determine AcP- and AcPb-dependent gene responses to IL-1 by virally-reconstituting AcP-deficient mouse embryonic cortical neurons with CD25 (control), full length AcP, AcPb or the combination of both. A control population was transduced with a CD25-expressing virus. Half the samples were stimulated with IL-1-beta for four hours, RNA was analyzed by microarray. Experiment Overall Design: Each in triplicate: CD25 cells, AcP cells, AcPb cells, AcP+AcPb cells. Cultures stimulated with IL-1b (10 ng/ml) for 4 hours then RNA was collected for processing and microarray analysis.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16104http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16104/samples/