ArrayExpress Uploader08412ad libitum chow during pregnancy and sucklingad libitum chow during pregnancy and sucklingad libitum chow during pregnancy and sucklingad libitum chow during pregnancy and sucklingad libitum chow during pregnancy and sucklingundernutrition during pregnancy, ad libitum chow during sucklingundernutrition during pregnancy, ad libitum chow during sucklingundernutrition during pregnancy, ad libitum chow during sucklingundernutrition during pregnancy, ad libitum chow during sucklingundernutrition during pregnancy, ad libitum chow during sucklingundernutrition during pregnancy and sucklingundernutrition during pregnancy and sucklingundernutrition during pregnancy and sucklingundernutrition during pregnancy and sucklingarrayexpress_sid8OBJECTIVE: To characterize the hormonal milieu and adipose gene expression in response to catch-up growth (CUG), a growth pattern...19208909E-GEOD-16083/profile/8773/arrayexpressuploader114adipocyteadipose tissuebodycellinsulinobesityserumDec.12, 2014Falsetranscription-profiling-by-array-of-mice-after-inE-GEOD-16083_A-AFFY-36Transcription profiling by array of mice after in utero undernutrition and/or undernutrition during sucklingNov.24, 2014OBJECTIVE: To characterize the hormonal milieu and adipose gene expression in response to catch-up growth (CUG), a growth pattern associated with obesity and diabetes risk, in a mouse model of low birth weight (LBW). RESEARCH DESIGN AND METHODS: ICR mice were food restricted by 50% from gestational days 12.5-18.5, reducing offspring birth weight by 25%. During the suckling period, dams were either fed ad libitum, permitting CUG in offspring, or food restricted, preventing CUG. Offspring were killed at age 3 weeks, and gonadal fat was removed for RNA extraction, array analysis, RT-PCR, and evaluation of cell size and number. Serum insulin, thyroxine (T4), corticosterone, and adipokines were measured. RESULTS: At age 3 weeks, LBW mice with CUG (designated U-C) had body weight comparable with controls (designated C-C); weight was reduced by 49% in LBW mice without CUG (designated U-U). Adiposity was altered by postnatal nutrition, with gonadal fat increased by 50% in U-C and decreased by 58% in U-U mice (P < 0.05 vs. C-C mice). Adipose expression of the lipogenic genes Fasn, AccI, Lpin1, and Srebf1 was significantly increased in U-C compared with both C-C and U-U mice (P < 0.05). Mitochondrial DNA copy number was reduced by >50% in U-C versus U-U mice (P = 0.014). Although cell numbers did not differ, mean adipocyte diameter was increased in U-C and reduced in U-U mice (P < 0.01). CONCLUSIONS: CUG results in increased adipose tissue lipogenic gene expression and adipocyte diameter but not increased cellularity, suggesting that catch-up fat is primarily associated with lipogenesis rather than adipogenesis in this murine model. Epididymal fat samples were obtained at age 3 weeks from 5 control mice (CC), 5 mice exposed to in utero undernutrition (UC), and 4 mice exposed to undernutrition in utero and during suckling (UU).http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16083mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16083/samples/