ArrayExpress Uploader0human1425arrayexpress_sid3In order to identify patterns of gene expression associated with biological effects in THP-1 cells induced by F3, we performed a...E-GEOD-16014/profile/8773/arrayexpressuploader06celldishDec.12, 2014Falsetranscription-profiling-of-human-monocytic-leukemiE-GEOD-16014_A-AFFY-33Transcription profiling of human monocytic leukemia cell line THP-1 treated with Ganoderma lucidum polysaccharides F3Jun.19, 2014In order to identify patterns of gene expression associated with biological effects in THP-1 cells induced by F3, we performed a transcriptomic analysis on the THP-1 control and F3-treated THP-1 cells by oligonucleotide microarray Experiment Overall Design: 10^7 cells/mL concentrations of THP-1 cells were seeded in 100 mm dish and incubated overnight. After that, cells were treated with F3 at a final concentration of 30 ug/mL. After incubated for 6 and 24 hours, the cell pellets were collected by centrifugation at 250g for 5 min, correspondingly. Controlled samples of un-induced cells were treated in the same way with the same amount of medium.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16014http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-16014/samples/