ArrayExpress Uploaderarrayexpress_sidmouse568406The Nuclear Factor I C (NFI-C) transcription factor has been implicated in TGF-β signaling, extracellular matrix deposition and skin...19752192E-GEOD-15871/profile/8773/arrayexpressuploader018fibroblastskinskin appendageDec.12, 2014FalseE-GEOD-15871_A-AFFY-45transcription-profiling-of-mouse-nfi-c-knock-out-eTranscription profiling of mouse NFI-C knock out embryonic fibroblastsNov.11, 2014The Nuclear Factor I C (NFI-C) transcription factor has been implicated in TGF-β signaling, extracellular matrix deposition and skin appendage pathologies. We performed a global gene expression analysis in NFI-C-/- and wild-type embryonic primary murine fibroblasts. This indicated that NFI-C acts mostly to repress gene expression in response to TGF-β1. Misregulated genes featured a prominent over-representation of regulators of connective tissue inflammation and repair. Experiment Overall Design: mRNAs were isolated from embryonic fibroblasts obtained from 3 wild-type and 3 NFI-C knock-out mice, and their levels were probed using microarrays. Prior to RNA extraction, fibroblast cultures were treated or not with TGF-β1 for 1 hour to examine the immediate response to the growth factor, or treated for 10 hours to assess the delayed response.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-15871http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-15871/samples/