{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSE15871GSM398437": {}}, {"GSE15871GSM398438": {}}, {"GSE15871GSM398439": {}}, {"GSE15871GSM398440": {}}, {"GSE15871GSM398441": {}}, {"GSE15871GSM398442": {}}, {"GSE15871GSM398443": {}}, {"GSE15871GSM398444": {}}, {"GSE15871GSM398445": {}}, {"GSE15871GSM398446": {}}, {"GSE15871GSM398447": {}}, {"GSE15871GSM398448": {}}, {"GSE15871GSM398449": {}}, {"GSE15871GSM398450": {}}, {"GSE15871GSM398451": {}}, {"GSE15871GSM398452": {}}, {"GSE15871GSM398453": {}}, {"GSE15871GSM398454": {}}], "id": 5684, "pop_total": 0, "platform": 6, "summary_wrapped": "The Nuclear Factor I C (NFI-C) transcription factor has been implicated in TGF-\u03b2 signaling, extracellular matrix deposition and skin...", "pubmed_id": 19752192, "geo_gse_id": "E-GEOD-15871", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 18, "tags": ["fibroblast", "skin", "skin appendage"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-15871_A-AFFY-45", "slug": "transcription-profiling-of-mouse-nfi-c-knock-out-e", "geo_gds_id": "", "name": "Transcription profiling of mouse NFI-C knock out embryonic fibroblasts", "created": "Nov.11, 2014", "summary": "The Nuclear Factor I C (NFI-C) transcription factor has been implicated in TGF-\u03b2 signaling, extracellular matrix deposition and skin appendage pathologies. We performed a global gene expression analysis in NFI-C-/- and wild-type embryonic primary murine fibroblasts. This indicated that NFI-C acts mostly to repress gene expression in response to TGF-\u03b21. Misregulated genes featured a prominent over-representation of regulators of connective tissue inflammation and repair. Experiment Overall Design: mRNAs were isolated from embryonic fibroblasts obtained from 3 wild-type and 3 NFI-C knock-out mice, and their levels were probed using microarrays. Prior to RNA extraction, fibroblast cultures were treated or not with TGF-\u03b21 for 1 hour to examine the immediate response to the growth factor, or treated for 10 hours to assess the delayed response.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-15871", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-15871/samples/"}