{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 5677, "factors": [{"GSE15750GSM395388": {}}, {"GSE15750GSM395389": {}}, {"GSE15750GSM395390": {}}, {"GSE15750GSM395391": {}}, {"GSE15750GSM395392": {}}, {"GSE15750GSM395393": {}}, {"GSE15750GSM395394": {}}, {"GSE15750GSM395395": {}}, {"GSE15750GSM395396": {}}, {"GSE15750GSM395397": {}}, {"GSE15750GSM395398": {}}, {"GSE15750GSM395399": {}}, {"GSE15750GSM395400": {}}, {"GSE15750GSM395401": {}}, {"GSE15750GSM395402": {}}, {"GSE15750GSM395403": {}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "CD8 T cells play a crucial role in immunity to infection and cancer.  They are maintained in constant numbers, but upon stimulation with...", "pubmed_id": 19494812, "geo_gse_id": "E-GEOD-15750", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 16, "tags": ["cancer", "cell", "fatty acid", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-cd8-t-cells-fro-2", "geo_id_plat": "E-GEOD-15750_A-AFFY-45", "name": "Transcription profiling of mouse CD8 T cells from two mouse strains (OTI-WT and OTI-TRAF6 knockout) at two timepoints", "created": "Nov.11, 2014", "summary": "CD8 T cells play a crucial role in immunity to infection and cancer.  They are maintained in constant numbers, but upon stimulation with antigen undergo a developmental program characterized by distinct phases encompassing the expansion and then contraction of antigen-specific populations, followed by the persistence of long-lived memory cells.  Although this predictable pattern of a CD8 T cell response is well established, the underlying cellular mechanisms regulating the transition to memory remain undefined.  Here we show that TRAF6, an adapter protein in the TNF-receptor (TNFR) and IL-1R/TLR superfamily, regulates CD8 T cell memory development following infection by modulating fatty acid metabolism. We show that mice with a T cell-specific deletion of TRAF6 mount robust primary CD8 T cell effector responses, but have a profound defect in their ability to generate memory. This defect is CD8 T cell intrinsic and is characterized by the disappearance of antigen-specific cells in the weeks following primary immunization.  Microarray analyses revealed that TRAF6-deficient CD8 T cells from early timepoints following immunization exhibit altered expression of genes that regulate fatty acid metabolism.  Consistent with this, activated CD8 T cells lacking TRAF6 are unable to upregulate mitochondrial \u03b2-oxidation in response to growth factor withdrawal in vitro.  Treatment with drugs that induce fatty acid oxidation enabled CD8 T cell memory generation in the absence of TRAF6.  Remarkably, these treatments also increased CD8 T cell memory in wild type mice, and consequently were able to significantly improve the efficacy of an experimental anti-cancer vaccine. Experiment Overall Design: CD8 T cells from two mouse strains (OTI-WT and OTI-TRAF6 knockout) at two timepoints (6d with 3 replicates and 10d with 5 replicates) after infection are used.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-15750", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-15750/samples/"}