{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 3252, "factors": [{"GSM394092": {"DOSAGE": "not specified"}}, {"GSM394092": {"DOSAGE": "not specified"}}, {"GSM394094": {"DOSAGE": "1 ng/ml"}}, {"GSM394095": {"DOSAGE": "100 ng/ml"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "NK cells are believed to contribute to the control of hepatitis C virus infection and pathogenesis of liver disease. Standard treatment...", "pubmed_id": 20334827, "geo_gse_id": "E-GEOD-15743", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 4, "tags": ["disease", "hepatitis", "hepatitis c", "liver", "liver disease", "protein", "serum", "virus infection"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "ifn-alpha-induced-gene-expression-in-human-nk-cell", "geo_id_plat": "E-GEOD-15743_A-AFFY-44", "name": "IFN alpha-induced gene expression in human NK cells", "created": "Sep.12, 2014", "summary": "NK cells are believed to contribute to the control of hepatitis C virus infection and pathogenesis of liver disease. Standard treatment of both acute and chronic hepatitis C is based on the administration of interferon alpha, however, the effects of type I interferons on human NK cells have not been studied in the context of hepatitis C. We therefore first performed a microarray screen for genes differentially regulated in human NK cells after stimulation of PBMC with recombinant interferon alpha-2b. One of the genes upregulated was TRAIL which was confirmed in vitro on the protein level.  Keywords: Interferon alpha; human NK cells; stimulation for 6 hours; cells sorted after stimulation of whole PBMC NK cells of PBMC of five healthy controls have been either isolated directly or after culturing for 6h in media containing 10% human AB serum supplemented with 1 and 100 ng/ml recombinant IFNa-2b. RNA of NK cells of the healthy controls was isolated and RNA was pooled for the hybridization.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-15743", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-15743/samples/"}