ArrayExpress Uploader0human1441arrayexpress_sid3Whereas estrogens exert their effects by binding to nuclear estrogen receptors and directly altering target gene transcription, they can...E-GEOD-15717/profile/8773/arrayexpressuploader09adenocarcinomabreastbreast adenocarcinomaestrogenDec.12, 2014Falsetranscription-profiling-of-human-mcf-7-breast-adenE-GEOD-15717_A-AFFY-33Transcription profiling of human MCF-7 breast adenocarcinoma cells were treated with empty dendrimer control, E2, or Estrogen Dendrimer Conjugate (EDC) for 4 hours to investigate nuclear and extranuclear pathway inputs in the regulation of global gene expression by estrogen receptorsJun.19, 2014Whereas estrogens exert their effects by binding to nuclear estrogen receptors and directly altering target gene transcription, they can also initiate extranuclear signaling through activation of kinase cascades. We have investigated the impact of estrogen-mediated extranuclear initiated pathways on global gene expression by using estrpgen-dendrimer conjugates. Experiment Overall Design: MCF-7 human breast adenocarcinoma cells were treated with empty dendrimer control, E2, or Estrogen Dendrimer Conjugate (EDC) for 4 hours, and cDNA microarray analyses were carried out using Affymetrix HG-U133A GeneChipshttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-15717http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-15717/samples/