ArrayExpress Uploader0mouse5667arrayexpress_sid6T cell differentiation to the Th17 effector subset requires stimulation through the T cell and co-stimulatory receptors, together with...E-GEOD-15624/profile/8773/arrayexpressuploader012amino acidcellcytokineDec.12, 2014Falsetranscription-profiling-of-mouse-t-cells-treated-wE-GEOD-15624_A-AFFY-45Transcription profiling of mouse T cells treated with halofuginone to investigate regulation of Y cell gene expression by Halofuginone (HF)Nov.11, 2014T cell differentiation to the Th17 effector subset requires stimulation through the T cell and co-stimulatory receptors, together with cytokine stimulation by TGFb and IL-6. The small molecule halofuginone (HF) inhibits Th17 cell development and induces a pattern of stress-regulated gene expression that mimics amino acid starvation. We used global transcript profiling to ask how halofuginone modulates gene expression induced during T cell activaiton and Th17 differentiation Experiment Overall Design: Purified mouse CD4+ CD25- T cells were activated under Th17 polarizing cytokine conditions and treated with either halofuginone or its inactive derivative, MAZ1310, for 3- or 6-hours.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-15624http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-15624/samples/