{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 3235, "factors": [{"GSE15481GSM388424": {}}, {"GSE15481GSM388425": {}}, {"GSE15481GSM388426": {}}, {"GSE15481GSM388427": {}}, {"GSE15481GSM388428": {}}, {"GSE15481GSM388429": {}}, {"GSE15481GSM388430": {}}, {"GSE15481GSM388431": {}}, {"GSE15481GSM388432": {}}, {"GSE15481GSM388433": {}}, {"GSE15481GSM388434": {}}, {"GSE15481GSM388435": {}}, {"GSE15481GSM388436": {}}, {"GSE15481GSM388437": {}}, {"GSE15481GSM388438": {}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Overexpression of the AP-2\u03b3  transcription factor in breast tumours has been identified as an independent predictor of poor outcome and...", "pubmed_id": 19798054, "geo_gse_id": "E-GEOD-15481", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 15, "tags": ["breast", "breast carcinoma", "carcinoma", "cell", "hormone", "line"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "sirna-of-human-mcf-7-cells", "geo_id_plat": "E-GEOD-15481_A-AFFY-44", "name": "siRNA of human MCF-7 cells", "created": "Sep.12, 2014", "summary": "Overexpression of the AP-2\u03b3  transcription factor in breast tumours has been identified as an independent predictor of poor outcome and failure of hormone therapy, even in ER positive, ErbB2 negative tumours; markers of a more favourable prognosis. To understand further the role of AP-2\u03b3 in breast carcinoma, we have used an RNA interference and gene expression profiling strategy using the MCF-7 cell line as a model for ER positive, ErbB2 negative tumours with AP-2\u03b3 overexpression. Gene expression changes between control and silenced cells implicate AP-2\u03b3  in the control of cell cycle progression and developmental signalling. Experiment Overall Design: We compared the expression profiles of MCF-7 cells separately transfected with three independent AP-2\u03b3 targeting sequences with those from control cells treated with transfection reagent alone or a non-silencing control siRNA on Affymetrix arrays; each condition was examined in triplicate.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-15481", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-15481/samples/"}