6ArrayExpress Uploader0mouseLow frequencies of switching in vitro36-65 hybridoma subclone L25Low frequencies of switching in vitro36-65 hybridoma subclone L27Spontaneous high frequencies of switching in vitro36-65 hybridoma subclone H23Spontaneous high frequencies of switching in vitro36-65 hybridoma subclone H275655arrayexpress_sidhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-15357This is a comparative microarray analysis of 36-65 hybridomas switching at normal low frequencies in vitro vs. selected variants.../profile/8773/arrayexpressuploader24chromatinclassDec.12, 2014FalseE-GEOD-15357_A-AFFY-45molecular-characterization-of-hybridoma-subclonesMolecular characterization of hybridoma subclones spontaneously switching at high frequencies in vitroNov.11, 2014This is a comparative microarray analysis of 36-65 hybridomas switching at normal low frequencies in vitro vs. selected variants spontaneously switching at 100 fold higher frequencies in vitro. This analysis reveals differential gene expression of 2091 unique probe sets, 1637 of them corresponded to known genes (647 of which were up-regulated and 990 down-regulated in the variants spontaneously switching at higher frequencies), including genes known to be involved in regular class switching (such as Cd40, Lig4, H2afx,Ung, Xrcc4, Tcfe2a, Id2, Apex1, Bcl6 and Prdm1) as well as genes involved in processes known to be necessary for regular class switch recombination (CSR), such as transcription, response to DNA damage and DNA repair, DNA binding, chromatin modifications, etc. Two subclones of each hybridoma switching at low (L) and high (H) frequencies were analyzed.E-GEOD-15357http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-15357/samples/