{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSE15267GSM381301": {}}, {"GSE15267GSM381302": {}}, {"GSE15267GSM381303": {}}, {"GSE15267GSM381304": {}}, {"GSE15267GSM381305": {}}, {"GSE15267GSM381306": {}}, {"GSE15267GSM381307": {}}, {"GSE15267GSM381308": {}}], "id": 5644, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "We present a robust serum-free system for the rapid and efficient reprogramming of mouse somatic cells by Oct4, Sox2 and Klf4.  The...", "geo_gse_id": "E-GEOD-15267", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 8, "tags": ["cell", "fibroblast", "pluripotent stem cell", "serum", "stem cell"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiing-of-mouse-induced-pluripoten", "geo_id_plat": "E-GEOD-15267_A-AFFY-45", "name": "Transcription profi;ing of mouse induced pluripotent stem cells vs MEF cells to investigate culturing conditions", "created": "Nov.11, 2014", "summary": "We present a robust serum-free system for the rapid and efficient reprogramming of mouse somatic cells by Oct4, Sox2 and Klf4.  The elimination of fetal bovine serum and oncogene c-Myc allowed reprogramming cells to be detected as early as Day 2 and reached greater than 10% of the population at Day 7 post retroviral transduction. The resulting iPS colonies were isolated with high efficiency to establish pluripotent cell lines. Based on this method, we further developed iPS-SF1 as a dedicated reprogramming medium for chemical screening and mechanistic investigations. Comparasion of iPS cell lines derived from serum-free culture condition, MEF cells cultured in serum and serum-free condition and ES cell lines was shown. Experiment Overall Design: Selected induced pluripotent stem cell lines and its original mouse embroynic fibroblast were comparaed to defined mouse embroynic stem cell lines: R1 and CGR8.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-15267", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-15267/samples/"}