4ArrayExpress Uploaderarrayexpress_sidhuman primary xenograftH446 H526 H1618 H1930 LX22 LX33 LX36 H69secondary xenograftH82secondary xenograftH128secondary xenograft primary xenograftH146secondary xenograftH187secondary xenograftH209secondary xenograftH345secondary xenograftH446secondary xenograftH526secondary xenograftH1618secondary xenograftH1930secondary xenograftLX22secondary xenograftLX33secondary xenograftH69 LX36secondary xenograft lung tissue reference RNA primary xenograftH128secondary xenograftLX36secondary xenograft reference RNA primary xenograftH82 H128 H146 H187 H209 H345 H1618 H187 H526 H82 32160http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-15240Gene expression was measured on the Affymetrix platform in primary xenografts, xenograft-derived cell lines, secondary xenografts, normal...19351829/profile/8773/arrayexpressuploader241cancercellgenomelunglung cancersclcDec.12, 2014Falsetranscription-profiling-by-array-of-human-xenografE-GEOD-15240_A-AFFY-44Transcription profiling by array of human xenograft laboratory models and primary tumors of small cell lung cancerSep.12, 2014Gene expression was measured on the Affymetrix platform in primary xenografts, xenograft-derived cell lines, secondary xenografts, normal lung, and primary tumors obtained from chemotherapy naive Small Cell Lung Cancer (SCLC). The SCLC primary xenografts were serially propagated in vivo in immunodeficient mice. Cell lines were derived from each xenograft and grown for 6 months using conventional tissue culture conditions. Secondary xenografts were obtained from cell cultures by re-implantation in immunodeficient mice. Such SCLC laboratory models were analyzed along with conventional SCLC cell lines and the derivative secondary xenografts, with normal lung and primary tumors, to assess irreversible gene expression changes induced by culturing conditions. Experiment Overall Design: SCLC primary xenografts were compared to the corresponding xenograft-derived cell lines, and to the secondary xenografts established from the cell lines using the Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. Gene expression from SCLC primary tumors was measured using the Affymetrix GeneChip Human Genome U133A 2.0 Array. 3 datasets: GSM380476-GSM380512, GSM380513-GSM380516, and GSM380517-GSM380520E-GEOD-15240http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-15240/samples/