ArrayExpress Uploaderarrayexpress_sidhumanCD2- plasmacytoid dendritic cellCD2- plasmacytoid dendritic cellCD2+ plasmacytoid dendritic cellCD2+ plasmacytoid dendritic cell964003Plasmacytoid dendritic cells (pDCs) are key regulators of anti-viral immunity. They rapidly secrete IFN-alpha and cross-present viral...19454677E-GEOD-15215/profile/8773/arrayexpressuploader04cancercelldendriticJan.17, 2015FalseE-GEOD-15215_A-AFFY-33cd2-distinguishes-two-subsets-of-human-plasmacytoiCD2 distinguishes two subsets of human plasmacytoid dendritic cells with distinct phenotype and functionsJan.17, 2015Plasmacytoid dendritic cells (pDCs) are key regulators of anti-viral immunity. They rapidly secrete IFN-alpha and cross-present viral antigens thereby launching adaptive immunity. Here we show that activated human pDCs inhibit replication of cancer cells, and kill them in a contact dependent fashion. Expression of CD2 distinguishes two pDC subsets with distinct phenotype and function. Both subsets secrete IFN-alpha and express Granzyme B and TRAIL. CD2high pDCs uniquely express lysozyme and can be found in tonsils and in tumors. Both subsets launch recall T cell response. However, CD2high pDCs secrete higher levels of IL12 p40, express higher levels of co-stimulatory molecule CD80 and are more efficient in triggering proliferation of naïve allogeneic T cells. Thus, human blood pDCs are composed of subsets with specific phenotype and functions. pDC subsets were isolated from two different donors. They were analyzed in two independent experiments.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-15215http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-15215/samples/