ArrayExpress Uploader0969824.7wk preterm25.4wk preterm25.6wk preterm27.1wk preterm29.9wk preterm33.0wk preterm33.3wk preterm33.9wk preterm35.7wk preterm35.9wk preterm36.6wk preterm24.1wk preeclamptic30.4wk preeclamptic30.6wk preeclamptic31.1wk preeclamptic31.7wk preeclamptic31.9wk preeclamptic32.0wk preeclamptic32.9wk preeclamptic33.0wk preeclamptic34.0wk preeclamptic35.9wk preeclamptic37.6wk preeclampticarrayexpress_sid3Preeclampsia (PE), which affects 4-8% of human pregnancies, causes significant maternal and neonatal morbidity and mortality. Within the...18818296E-GEOD-14722/profile/8773/arrayexpressuploader023basaldiseasepreeclampsiaproteinuterusvasculatureJan.17, 2015Falsesevere-preeclampsia-related-changes-in-gene-expresE-GEOD-14722_A-AFFY-33Severe Preeclampsia-Related Changes in Gene Expression at the Maternal-Fetal Interface Include Siglec-6 and Pappalysin-2Jan.17, 2015Preeclampsia (PE), which affects 4-8% of human pregnancies, causes significant maternal and neonatal morbidity and mortality. Within the basal plate, placental cytotrophoblasts (CTBs) of fetal origin invade the uterus and extensively remodel the maternal vasculature. In PE, CTB invasion is often shallow, and vascular remodeling is rudimentary. To better understand possible causes, we conducted a global analysis of gene expression at the maternal-fetal interface in placental samples from women with PE (n = 12; 24-36 wk) vs. samples from women who delivered due to preterm labor with no evidence of infection (n = 11; 24-36 wk), a condition that our previous work showed is associated with normal CTB invasion. Using the HG-U133A&B Affymetrix GeneChip platform, and statistical significance set at log odds-ratio of B >0, 55 genes were differentially expressed in PE. They encoded proteins previously associated with PE [e.g. Flt-1 (vascular endothelial growth factor receptor-1), leptin, CRH, and inhibin] and novel molecules [e.g. sialic acid binding Ig-like lectin 6 (Siglec-6), a potential leptin receptor, and pappalysin-2 (PAPP-A2), a protease that cleaves IGF-binding proteins]. We used quantitative PCR to validate the expression patterns of a subset of the genes. At the protein level, we confirmed PE-related changes in the expression of Siglec-6 and PAPP-A2, which localized to invasive CTBs and syncytiotrophoblasts. Notably, Siglec-6 placental expression is uniquely human, as is spontaneous PE. The functional significance of these novel observations may provide new insights into the pathogenesis of PE, and assaying the circulating levels of these proteins could have clinical utility for predicting and/or diagnosing PE. Keywords: disease state analysis Basal plate biopsies of preterm labor (24-36 weeks; n=11) and preterm severe preeclampsia (24-36 weeeks; n=12) were isolated and the global gene expression profiles determined using Affymetrix Human GeneChips. Comparisons between the preeclampsia samples and the preterm labor controls revealed genes differentially expressed in preeclampsia.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14722humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14722/samples/