{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM366905": {"STRAIN OR LINE": "C57BL/6, Tissue: Spleen, Mouse number: pooled cells from 10 mice"}}, {"GSM366907": {"STRAIN OR LINE": "C57BL/6, Tissue: Sacral and pancreatic lymph node, Mouse number: pooled cells from 20 mice"}}, {"GSM366905": {"STRAIN OR LINE": "C57BL/6, Tissue: Spleen, Mouse number: pooled cells from 10 mice"}}, {"GSM366905": {"STRAIN OR LINE": "C57BL/6, Tissue: Spleen, Mouse number: pooled cells from 10 mice"}}, {"GSM366905": {"STRAIN OR LINE": "C57BL/6, Tissue: Spleen, Mouse number: pooled cells from 10 mice"}}, {"GSM366907": {"STRAIN OR LINE": "C57BL/6, Tissue: Sacral and pancreatic lymph node, Mouse number: pooled cells from 20 mice"}}, {"GSM366905": {"STRAIN OR LINE": "C57BL/6, Tissue: Spleen, Mouse number: pooled cells from 10 mice"}}], "id": 5618, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Peripheral tolerance induction is critical for the maintenance of self-tolerance and can be mediated by immunoregulatory T cells or by...", "pubmed_id": 19204323, "geo_gse_id": "E-GEOD-14699", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 7, "tags": ["cell", "il-7r", "lymphopenia", "peripheral", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-14699_A-AFFY-45", "slug": "expression-data-from-cd8-t-cells-undergoing-deleti", "geo_gds_id": "", "name": "Expression data from CD8+ T cells undergoing deletional tolerance", "created": "Nov.10, 2014", "summary": "Peripheral tolerance induction is critical for the maintenance of self-tolerance and can be mediated by immunoregulatory T cells or by direct induction of T cell anergy or deletion. While the molecular processes underlying anergy have been extensively studied, little is known about the molecular basis for peripheral T cell deletion. Here, we determined the gene expression signature of peripheral CD8+ T cells undergoing deletional tolerance, relative to those undergoing immunogenic priming or lymphopenia-induced proliferation. From these data, we report the first detailed molecular signature of cells undergoing deletion. Consistent with defective cytolysis, these cells exhibited deficiencies in granzyme up-regulation. Furthermore, they showed antigen-driven Bcl-2 down-regulation and early up-regulation of the pro-apoptotic protein Bim, consistent with the requirement of this BH3-only protein for peripheral T cell deletion. Bim up-regulation was paralleled by defective IL-7Ra chain re-expression, suggesting that Bim-dependent death may be triggered by loss of IL-7/IL-7R signaling. Finally, we observed parallels in molecular signatures between deletion and anergy suggesting that these tolerance pathways may not be as molecularly distinct as previously surmised. Na\u00efve (CD44lo) OT-I T cells were CFSE labelled and transferred in a model of deletional tolerance (RIP-OVAhi mice), a model of immunity (mice primed with OVA coated splenocytes and LPS) or a model of lymphopenia induced proliferation (Rag-/- mice).  60 hrs (RIP-OVAhi and OVA coated splenocytes) or 5 days (Rag-/-) after transfer, OT-I cells that had undergone two or more divisions as determined by CFSE dilution were sorted, RNA extracted and samples were prepared for hybridisation to Affymetrix microarrays.  As a control for naive cells, CFSE labelled OT-I cells were injected into antigen-free B6 mice and the undivided naive cells were sorted after 60 hrs and also used for microarray analysis.  Two replicates were prepared for the naive cells, cells from RIP-OVAhi mice and cells from OVA coated splenocyte primed mice, while one replicate was prepared for the cells from Rag-/- mice.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14699", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14699/samples/"}