ArrayExpress Uploader05614arrayexpress_sid6GM-CSF controls the development of granulocytes but little is known about the contribution of the downstream mediating transcription...19779039E-GEOD-14672/profile/8773/arrayexpressuploader012cellperipheralDec.12, 2014Falsetranscription-profiling-of-mouse-wild-type-and-staE-GEOD-14672_A-AFFY-45Transcription profiling of mouse wild type and Stat5a or 5b knock-out blood cells treated with GM-CSF to induce granulopoiesisNov.10, 2014GM-CSF controls the development of granulocytes but little is known about the contribution of the downstream mediating transcription factor STAT5A/B. To elucidate this pathway, we generated mice lacking the Stat5a and 5b genes in blood cells. Peripheral neutrophils were decreased and administration of 5-FU and GM-CSF failed to induce granulopoiesis in Stat5a/b-mutant mice. GMPs were isolated and cultured with GM-CSF. Both the number and size of STAT5A/B-null colonies were reduced and GM-CSF-induced survival of mature STAT5A/B-null neutrophils was impaired. Time-lapse cinematography and single cell tracking of GMPs revealed that STAT5A/B-null cells were characterized by a longer generation time and an increased cell death. Gene expression profiling experiments suggested that STAT5A/B directs GM-CSF signaling through the regulation of cell survival genes. Experiment Overall Design: Mice lacking or with the Stat5a and 5b genes in blood cells, which were treated w/o GMPhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14672mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14672/samples/