{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 5614, "factors": [{"GSE14672GSM366044": {}}, {"GSE14672GSM366045": {}}, {"GSE14672GSM366046": {}}, {"GSE14672GSM366047": {}}, {"GSE14672GSM366048": {}}, {"GSE14672GSM366049": {}}, {"GSE14672GSM366050": {}}, {"GSE14672GSM366051": {}}, {"GSE14672GSM366052": {}}, {"GSE14672GSM366053": {}}, {"GSE14672GSM366054": {}}, {"GSE14672GSM366055": {}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "GM-CSF controls the development of granulocytes but little is known about the contribution of the downstream mediating transcription...", "pubmed_id": 19779039, "geo_gse_id": "E-GEOD-14672", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 12, "tags": ["cell", "peripheral"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-wild-type-and-sta", "geo_id_plat": "E-GEOD-14672_A-AFFY-45", "name": "Transcription profiling of mouse wild type and Stat5a or 5b knock-out blood cells treated with GM-CSF to induce granulopoiesis", "created": "Nov.10, 2014", "summary": "GM-CSF controls the development of granulocytes but little is known about the contribution of the downstream mediating transcription factor STAT5A/B.  To elucidate this pathway, we generated mice lacking the Stat5a and 5b genes in blood cells.  Peripheral neutrophils were decreased and administration of 5-FU and GM-CSF failed to induce granulopoiesis in Stat5a/b-mutant mice. GMPs were isolated and cultured with GM-CSF.  Both the number and size of STAT5A/B-null colonies were reduced and GM-CSF-induced survival of mature STAT5A/B-null neutrophils was impaired.  Time-lapse cinematography and single cell tracking of GMPs revealed that STAT5A/B-null cells were characterized by a longer generation time and an increased cell death.  Gene expression profiling experiments suggested that STAT5A/B directs GM-CSF signaling through the regulation of cell survival genes. Experiment Overall Design: Mice lacking or with  the Stat5a and 5b genes in blood cells, which were treated w/o GMP", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14672", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14672/samples/"}