{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM366057 1": {}}, {"GSM366058 1": {}}, {"GSM366059 1": {}}, {"GSM366060 1": {}}, {"GSM366061 1": {}}, {"GSM366062 1": {}}, {"GSM366063 1": {}}, {"GSM366064 1": {}}, {"GSM366065 1": {}}, {"GSM366066 1": {}}, {"GSM366067 1": {}}, {"GSM366068 1": {}}, {"GSM366069 1": {}}, {"GSM366070 1": {}}, {"GSM366071 1": {}}, {"GSM366072 1": {}}], "id": 3159, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Background:  The dramatic clinical course of acute liver failure has posed major limitations for pathogenesis studies. We used gene...", "geo_gse_id": "E-GEOD-14668", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 16, "tags": ["cell", "hepatitis", "hepatitis b", "immunoglobulin", "liver"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "b-cell-gene-signature-in-hbv-associated-acute-live", "geo_id_plat": "E-GEOD-14668_A-AFFY-44", "name": "B-Cell Gene Signature in HBV-Associated Acute Liver Failure", "created": "Sep.11, 2014", "summary": "Background:  The dramatic clinical course of acute liver failure has posed major limitations for pathogenesis studies. We used gene expression profiling to establish a molecular definition of hepatitis B virus (HBV)-associated acute liver failure. Methods:  Two patients who underwent liver transplantation for HBV-associated acute liver failure were studied. Gene expression profiling was performed on 8 liver specimens obtained from the two patients with acute liver failure (4 samples per liver) and individual liver specimens from 8 liver donors. Statistical analyses were used to identify the signature genes of HBV-associated acute liver failure. Results:  Multivariate permutation analysis identified 1,368 transcripts that were differentially expressed in acute liver failure; 709 were up-regulated and 659 down-regulated. The most represented up-regulated transcripts were those involved in the immune response, whereas the most abundant down-regulated transcripts were those involved in metabolism and hepatic synthesis. Acute liver failure was characterized by an overriding B-cell signature comprising genes related to mature B cells and plasma cells with abundant polyclonal expression of immunoglobulin genes. By contrast, there was a limited T-cell signature and up-regulation of several inhibitors of T-cell activation. Immunohistochemical analysis confirmed the prominent B-cell signature showing diffuse liver infiltration by plasma blasts and plasma cells with strong cytoplasmic staining for IgM and IgG, associated with a significant deposition of complement factors. Conclusions:  The presence of a prominent intrahepatic plasma-cell infiltrate with ectopic immunoglobulin production and complement deposition suggests a pivotal role of humoral immunity in the pathogenesis of acute liver failure associated with HBV infection.  Keywords: Expression prrofiling by array Liver samples were obtained from  explanted livers of two patients with HBV-associated acute liver failure (4 samples per liver) and 8 individual normal liver donors and gene expression profiling was used to establish a molecular definition of acute liver failure.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14668", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14668/samples/"}