{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "human", "factors": [{"GSE14491GSM361954": {}}, {"GSE14491GSM361955": {}}, {"GSE14491GSM361956": {}}, {"GSE14491GSM361957": {}}, {"GSE14491GSM361958": {}}, {"GSE14491GSM361959": {}}, {"GSE14491GSM361960": {}}, {"GSE14491GSM361961": {}}, {"GSE14491GSM361962": {}}, {"GSE14491GSM361963": {}}, {"GSE14491GSM361964": {}}, {"GSE14491GSM361965": {}}, {"GSE14491GSM361966": {}}, {"GSE14491GSM361967": {}}, {"GSE14491GSM361968": {}}, {"GSE14491GSM361969": {}}], "id": 3149, "pop_total": 0, "platform": 4, "summary_wrapped": "TGF\u03b2 ligands act as tumor suppressors in early stage tumors but are paradoxically diverted into potent prometastatic factors in advanced...", "pubmed_id": 19345189, "geo_gse_id": "E-GEOD-14491", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 16, "tags": ["breast", "breast cancer", "cancer", "cell", "left"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-14491_A-AFFY-44", "slug": "shrnai-profiling-of-human-mda-mb-231-cells-express", "geo_gds_id": "", "name": "shRNAi profiling of human MDA-MB-231 cells expressing  anti-p53 (shp53) short-hairpin RNAs treated with TFGbeta or control", "created": "Sep.11, 2014", "summary": "TGF\u03b2 ligands act as tumor suppressors in early stage tumors but are paradoxically diverted into potent prometastatic factors in advanced cancers. The molecular nature of this switch remains enigmatic. We now show that TGF\u03b2-dependent cell migration, invasion and metastasis are empowered by mutant-p53. To investigate the specific gene expression program by which mutant-p53 and TGF\u03b2 control invasion and metastasis in breast cancer cells, we compared the TGF\u03b2 transcriptomic profile of control and mutant-p53 depleted MDA-MB-231 cells. Experiment Overall Design: MDA-MB-231 cells, stably expressing either control (shGFP) or anti-p53 (shp53) short-hairpin RNAs, were left untreated or treated with TGFbeta. Samples were then processed for total RNA extraction and hybridization on Affymetrix microarrays. Four biological replicas (A, B, C, D) were used for each of the 4 conditions (1: untreated control; 2: TGFbeta treated control; 3: untreated p53-depleted cells; 4: TGFbeta treated mutant-p53-depleted cells), for a total of 16 samples.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14491", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14491/samples/"}