ArrayExpress Uploaderarrayexpress_sidmouse560506Synovial sarcoma-like tumors were generated in mice by conditionally expressing the human t(X;18) translocation-derived SYT-SSX2 fusion...E-GEOD-14469/profile/8773/arrayexpressuploader05proteinsarcomasynovial sarcomaDec.12, 2014Falsetranscription-profiling-of-synovial-sarcoma-like-tE-GEOD-14469_A-AFFY-45Transcription profiling of synovial sarcoma-like tumors induced in a genetically engineered mouse modelNov.10, 2014Synovial sarcoma-like tumors were generated in mice by conditionally expressing the human t(X;18) translocation-derived SYT-SSX2 fusion protein. Using a Tamoxifen-inducible CreER system, we show here that sporadic expression of SYT-SSX2 across multiple tissue types leads to exclusive formation of synovial sarcoma-like tumors while its widespread expression is lethal. CreER-based sporadic expression both avoids the severe early developmental phenotypes associated with widespread SYT-SSX2 expression and better models natural pathogenesis of cancers where transformed cells usually arise within an environment of largely normal cells. Experiment Overall Design: Genetically engineered mice capable of conditionally expressing the human synovial sarcoma-associated SYT-SSX2 fusion oncogene were mated with genetically engineered mice expressing the CreER fusion protein from ROSA locus. The progenies harboring both CreER and SYT-SSX2 were followed up with or without tamoxifen injection. Tumors were generated in these mice that were dissected out, RNA extracted, and subjected to expression profiling by microarray analysis.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14469http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14469/samples/