{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSE14469GSM361386": {}}, {"GSE14469GSM361387": {}}, {"GSE14469GSM361388": {}}, {"GSE14469GSM361389": {}}, {"GSE14469GSM361390": {}}], "id": 5605, "pop_total": 0, "platform": 6, "summary_wrapped": "Synovial sarcoma-like tumors were generated in mice by conditionally expressing the human t(X;18) translocation-derived SYT-SSX2 fusion...", "geo_gse_id": "E-GEOD-14469", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 5, "tags": ["protein", "sarcoma", "synovial sarcoma"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-synovial-sarcoma-like-t", "geo_id_plat": "E-GEOD-14469_A-AFFY-45", "name": "Transcription profiling of synovial sarcoma-like tumors induced in a genetically engineered mouse model", "created": "Nov.10, 2014", "summary": "Synovial sarcoma-like tumors were generated in mice by conditionally expressing the human t(X;18) translocation-derived SYT-SSX2 fusion protein. Using a Tamoxifen-inducible CreER system, we show here that sporadic expression of SYT-SSX2 across multiple tissue types leads to exclusive formation of synovial sarcoma-like tumors while its widespread expression is lethal.  CreER-based sporadic expression both avoids the severe early developmental phenotypes     associated with widespread SYT-SSX2 expression and better models natural pathogenesis of cancers where transformed cells usually arise within an environment of largely normal cells. Experiment Overall Design: Genetically engineered mice capable of conditionally expressing the human synovial sarcoma-associated SYT-SSX2 fusion oncogene were mated with genetically engineered mice expressing the CreER fusion protein from ROSA locus. The progenies harboring both CreER and SYT-SSX2 were followed up with or without tamoxifen injection. Tumors were generated in these mice that were dissected out, RNA extracted, and subjected to expression profiling by microarray analysis.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14469", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14469/samples/"}