ArrayExpress Uploader0rat9040arrayexpress_sid11TNF-a is increased in the synovial fluid of patients with rheumatoid arthritis and osteoarthritis. TNF-a activates MEK/ERK in...E-GEOD-14402/profile/8773/arrayexpressuploader08arthritisosteoarthritisrheumatoid arthritissynovial fluidFeb.09, 2015Falsetranscription-profiling-of-rat-chondrocytes-were-tE-GEOD-14402_A-AFFY-43Transcription profiling of rat chondrocytes were treated with TNF-a with or without the MEK1/2 inhibitor U0126 for 24 h to investigate TNF-a-induced MEK/ERK-dependent regulation of Cartilage Matrix GenesJan.08, 2015TNF-a is increased in the synovial fluid of patients with rheumatoid arthritis and osteoarthritis. TNF-a activates MEK/ERK in chondrocytes; however the overall functional relevance of MEK/ERK to TNF-a-regulated gene expression in chondrocytes is unknown. Chondrocytes were treated with TNF-a with or without the MEK1/2 inhibitor U0126 for 24 h. Microarray analysis was used to identify genes regulated by TNF-a in a MEK1/2-dependent fashion. Experiment Overall Design: Primary chondrocytes from the femoral condyles of neonatal rats were treated with DMSO (control sample), TNF-a, U0126 (selective MEK1/2 inhibitor) or TNF-a and U0126. Two biological replicates were collected for each treatment. Total RNA was collected and analyzed by Affymetrix Microarray.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14402http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14402/samples/