{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "rat", "factors": [{"GSE14402GSM359894": {}}, {"GSE14402GSM359894": {}}, {"GSE14402GSM359894": {}}, {"GSE14402GSM359894": {}}, {"GSE14402GSM359894": {}}, {"GSE14402GSM359894": {}}, {"GSE14402GSM359894": {}}, {"GSE14402GSM359894": {}}], "id": 9040, "ownerprofile_id": "arrayexpress_sid", "platform": 11, "summary_wrapped": "TNF-a is increased in the synovial fluid of patients with rheumatoid arthritis and osteoarthritis.  TNF-a activates MEK/ERK in...", "geo_gse_id": "E-GEOD-14402", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 8, "tags": ["arthritis", "osteoarthritis", "rheumatoid arthritis", "synovial fluid"], "lastmodified": "Feb.09, 2015", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-rat-chondrocytes-were-t", "geo_id_plat": "E-GEOD-14402_A-AFFY-43", "name": "Transcription profiling of rat chondrocytes were treated with TNF-a with or without the MEK1/2 inhibitor U0126 for 24 h to investigate TNF-a-induced MEK/ERK-dependent regulation of Cartilage Matrix Genes", "created": "Jan.08, 2015", "summary": "TNF-a is increased in the synovial fluid of patients with rheumatoid arthritis and osteoarthritis.  TNF-a activates MEK/ERK in chondrocytes; however the overall functional relevance of MEK/ERK to TNF-a-regulated gene expression in chondrocytes is unknown. Chondrocytes were treated with TNF-a with or without the MEK1/2 inhibitor U0126 for 24 h. Microarray analysis was used to identify genes regulated by TNF-a in a MEK1/2-dependent fashion. Experiment Overall Design: Primary chondrocytes from the femoral condyles of neonatal rats were treated with DMSO (control sample), TNF-a, U0126 (selective MEK1/2 inhibitor) or TNF-a and U0126.  Two biological replicates were collected for each treatment.  Total RNA was collected and analyzed by Affymetrix Microarray.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14402", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14402/samples/"}