ArrayExpress Uploader0mouseHunk knockoutHunk knockoutHunk knockoutHunk knockoutHunk knockoutHunk knockoutwild type genotypewild type genotypewild type genotypewild type genotypewild type genotypewild type genotype8230arrayexpress_sid7We previously identified a novel SNF1/AMPK-related protein kinase, Hunk, from a mammary tumor arising in an MMTV-neu transgenic mouse....19717424E-GEOD-14226/profile/8773/arrayexpressuploader112breastcancercolonmammary tumorovaryproteinDec.12, 2014Falsetranscription-profiling-by-array-of-mammary-glandE-GEOD-14226_A-AFFY-23Transcription profiling by array of mammary gland tissues from Hunk (a novel SNF1/AMPK-related protein kinase) knockout and wild type controlsNov.23, 2014We previously identified a novel SNF1/AMPK-related protein kinase, Hunk, from a mammary tumor arising in an MMTV-neu transgenic mouse. The function of this kinase is unknown. Using targeted deletion in mice, we now demonstrate that Hunk is required for the metastasis of c-myc-induced mammary tumors, but is dispensable for normal development. Reconstitution experiments revealed that Hunk is sufficient to restore the metastatic potential of Hunk-deficient tumor cells, as well as defects in migration and invasion, and does so in a manner that requires its kinase activity. Consistent with a role for Hunk in the progression of human cancers, the human homologue of Hunk is overexpressed in aggressive subsets of carcinomas of the ovary, colon, and breast. In addition, a murine gene expression signature that distinguishes Hunk-wild type from Hunk-deficient mammary tumors predicts clinical outcome in women with breast cancer. Together, these findings establish a role for Hunk in metastasis and an in vivo function for this kinase. Hunk-deficient animals were crossed to mice harboring an MMTV-c-myc transgene (Leder et al., 1986). Hunk heterozygous, MMTV-c-myc mice were backcrossed to Hunk heterozygous animals. MMTV-c-myc female animals of each Hunk genotype were mated twice, then monitored twice weekly for mammary tumors. Mice possessing tumors with a maximum diameter of 20 mm were sacrificed and organs were examined at necropsy. Tumor nodules were identified by examination of organs through a Leica Wild MZ8 dissection microscope.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14226http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14226/samples/