{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM356505": {"genotype": "Hunk knockout"}}, {"GSM356505": {"genotype": "Hunk knockout"}}, {"GSM356505": {"genotype": "Hunk knockout"}}, {"GSM356505": {"genotype": "Hunk knockout"}}, {"GSM356505": {"genotype": "Hunk knockout"}}, {"GSM356505": {"genotype": "Hunk knockout"}}, {"GSM3565": {"genotype": "wild type genotype"}}, {"GSM3565": {"genotype": "wild type genotype"}}, {"GSM3565": {"genotype": "wild type genotype"}}, {"GSM3565": {"genotype": "wild type genotype"}}, {"GSM3565": {"genotype": "wild type genotype"}}, {"GSM3565": {"genotype": "wild type genotype"}}], "id": 8230, "ownerprofile_id": "arrayexpress_sid", "platform": 7, "summary_wrapped": "We previously identified a novel SNF1/AMPK-related protein kinase, Hunk, from a mammary tumor arising in an MMTV-neu transgenic mouse....", "pubmed_id": 19717424, "geo_gse_id": "E-GEOD-14226", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 12, "tags": ["breast", "cancer", "colon", "mammary tumor", "ovary", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-by-array-of-mammary-gland", "geo_id_plat": "E-GEOD-14226_A-AFFY-23", "name": "Transcription profiling by array of mammary gland tissues from Hunk (a novel SNF1/AMPK-related protein kinase) knockout and wild type controls", "created": "Nov.23, 2014", "summary": "We previously identified a novel SNF1/AMPK-related protein kinase, Hunk, from a mammary tumor arising in an MMTV-neu transgenic mouse. The function of this kinase is unknown. Using targeted deletion in mice, we now demonstrate that Hunk is required for the metastasis of c-myc-induced mammary tumors, but is dispensable for normal development. Reconstitution experiments revealed that Hunk is sufficient to restore the metastatic potential of Hunk-deficient tumor cells, as well as defects in migration and invasion, and does so in a manner that requires its kinase activity. Consistent with a role for Hunk in the progression of human cancers, the human homologue of Hunk is overexpressed in aggressive subsets of carcinomas of the ovary, colon, and breast. In addition, a murine gene expression signature that distinguishes Hunk-wild type from Hunk-deficient mammary tumors predicts clinical outcome in women with breast cancer. Together, these findings establish a role for Hunk in metastasis and an in vivo function for this kinase. Hunk-deficient animals were crossed to mice harboring an MMTV-c-myc transgene (Leder et al., 1986).  Hunk heterozygous, MMTV-c-myc mice were backcrossed to Hunk heterozygous animals.  MMTV-c-myc female animals of each Hunk genotype were mated twice, then monitored twice weekly for mammary tumors.  Mice possessing tumors with a maximum diameter of 20 mm were sacrificed and organs were examined at necropsy.  Tumor nodules were identified by examination of organs through a Leica Wild MZ8 dissection microscope.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14226", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14226/samples/"}