{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 5585, "factors": [{"GSM356329": {"SALL4": "flox/-", "FEEDER CELLS": "absent"}}, {"GSM356330": {"SALL4": "delta/-", "FEEDER CELLS": "absent"}}, {"GSM35633": {"SALL4": "flox/-", "FEEDER CELLS": "present"}}, {"GSM356332": {"SALL4": "delta/-", "FEEDER CELLS": "present"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Sall4 is a mouse homolog of a causative gene of the autosomal dominant disorder known as Okihiro syndrome. We previously showed that...", "pubmed_id": 19350679, "geo_gse_id": "E-GEOD-14219", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 4, "tags": ["cell", "fibroblast", "syndrome"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-profile-of-sall4-null-es-cells-and-sall", "geo_id_plat": "E-GEOD-14219_A-AFFY-45", "name": "Expression profile of Sall4-null ES cells and Sall4 heterozygous ES cells", "created": "Nov.10, 2014", "summary": "Sall4 is a mouse homolog of a causative gene of the autosomal dominant disorder known as Okihiro syndrome. We previously showed that Sall4 absence leads to lethality during peri-implantation and that Sall4-null embryonic stem (ES) cells proliferate poorly with intact pluripotency when cultured on feeder cells. However, a subsequent report indicated that shRNA-mediated Sall4 inhibition in ES cells led to a severe reduction in Oct3/4 and a secondary increase in Cdx2, which resulted in complete differentiation into the trophectoderm when cultured in the feeder-free condition. So we profiled gene expression changes when Sall4 is deleted in ES cells in the presence or absence of feeder cells.  key word: embryonic stem (ES) cell, Sall4, feeder ES cells were cultured with or without mouse embryonic fibroblast (MEF) feeder cells in LIF-supplemented medium as described. To maintain the expression of Oct3/4, all ES cells were cultured in the presence of Blasticidin.  Four samples were analyzed.  GSM356329, GSM356330 : cultured in the absence of feeders GSM356331, GSM356332 : cultured on the feeders", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14219", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-14219/samples/"}