{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSE13806GSM347160": {}}, {"GSE13806GSM347161": {}}, {"GSE13806GSM347162": {}}, {"GSE13806GSM347163": {}}, {"GSE13806GSM347164": {}}, {"GSE13806GSM347165": {}}, {"GSE13806GSM347166": {}}, {"GSE13806GSM347167": {}}, {"GSE13806GSM347168": {}}, {"GSE13806GSM347169": {}}, {"GSE13806GSM347170": {}}, {"GSE13806GSM347171": {}}, {"GSE13806GSM347172": {}}, {"GSE13806GSM347173": {}}, {"GSE13806GSM347174": {}}, {"GSE13806GSM347175": {}}, {"GSE13806GSM347176": {}}], "id": 3105, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "RNA was isolated from ectopically sFRP1-expressing MDA-MB-231 cells and control MDA-MB-231 cells and as well from tumor lysates arising...", "geo_gse_id": "E-GEOD-13806", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 17, "tags": ["breast", "breast cancer", "cancer"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-human-mda-mb-231-breast", "geo_id_plat": "E-GEOD-13806_A-AFFY-44", "name": "Transcription profiling of human MDA-MB-231 breast cancer cells in vitro and in vivo as xenografts to assess the effect of ectopic expression of sFRP1", "created": "Sep.11, 2014", "summary": "RNA was isolated from ectopically sFRP1-expressing MDA-MB-231 cells and control MDA-MB-231 cells and as well from tumor lysates arising from these cells as nude mouse xenograft.  Gene expression profiles for these samples were investigated using Affymetrix arrays. Experiment Overall Design: MDA-MB-231 human breast cancer cells were stably transfected with human sFRP1 encoding vector or empty vector as control. After the selection with antibiotics, three clones of MDA-MB-231/sFRP1 and three clones of MDA-MB-231/control were selected. These six clones were cultured individually in DMEM 10% FCS with 1mg/ml G-418. When cells reached 70-80% confluence, RNA was isolated from the cells. In parallel, the three clones of MDA-MB-231/sFRP1 and the three clones of MDA-MB-231/control were pooled respectively. One million of cells from each pool were suspended in 100ul PBS and injected to fat pads of female balb/c nude mice (6 mice were injected with MDA-MB-231/sFRP1 and 5 mice were injected with MDA-MB-231/control) to do a xenograft experiment. A few - several weeks after, mice were sacrificed when tumor reached a certain size, tumors were taken and RNA was isolated using trizol reagent.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13806", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13806/samples/"}