4ArrayExpress Uploader0human0 CD14-positive monocytenone5 immature dendritic cellvehicle510immature dendritic cell1,25-dihydroxyvitamin D3 12 differentiating dendritic cellvehicle1210differentiating dendritic cell1,25-dihydroxyvitamin D3 0 CD14-positive monocytenone5 immature dendritic cellvehicle510immature dendritic cell1,25-dihydroxyvitamin D3 12 differentiating dendritic cellvehicle1210differentiating dendritic cell1,25-dihydroxyvitamin D3 0 CD14-positive monocytenone5 immature dendritic cellvehicle510immature dendritic cell1,25-dihydroxyvitamin D3 12 differentiating dendritic cellvehicle1210differentiating dendritic cell1,25-dihydroxyvitamin D3 3103arrayexpress_sidhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13762We have carried out global gene expression analysis to clarify the interrelationship between 1,25-dihydroxyvitamin D3 and...19201860/profile/8773/arrayexpressuploader415celldendriticgenomemonocyteDec.12, 2014Falsetranscription-profiling-by-array-of-human-monocy-3E-GEOD-13762_A-AFFY-44Transcription profiling by array of human monocyte-derived dendritic cells treated with 1,25-dihydroxyvitamin D3Sep.11, 2014We have carried out global gene expression analysis to clarify the interrelationship between 1,25-dihydroxyvitamin D3 and differentiation-driven gene expression patterns in developing human monocyte-derived dendritic cells. Monocytes were treated with 10 nM 1,25-dihydroxyvitamin D3 or vehicle 14 hours after plating for 12 hours or 5 days. Monocytes, differentiating dendritic cells (+/-1,25-dihydroxyvitamin D3 for 12 hours) and immature dendritic cells (+/-1,25-dihydroxyvitamin D3 for 5 days) were harvested. This design allows one to identify genes regulated by differentiation and/or 1,25-dihydroxyvitamin D3 in human monocyte-derived dendritic cells. Experiment Overall Design: Human monocytes were obtained from buffy coats from healthy donors by Ficoll gradient centrifugation followed by immunomagnetic cell separation with anti-CD14-conjugated microbeads. Monocytes were cultured in RPMI-1640 supplemented with 10% FBS, 800 U/ml GM-CSF and 500 U/ml IL-4. Monocytes were treated with 10 nM 1,25-dihydroxyvitamin D3 or vehicle 14 hours after plating for 12 hours or 5 days. Monocytes, differentiating dendritic cells (+/-1,25-dihydroxyvitamin D3 for 12 hours) and immature dendritic cells (+/-1,25-dihydroxyvitamin D3 for 5 days) were harvested. Experiments were performed in biological triplicates representing samples from different donors. 15 samples were processed and hybridized to Human Genome U133 Plus 2.0 Arrays.E-GEOD-13762http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-13762/samples/